红丝线药材的HPLC指纹图谱研究
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篇名: 红丝线药材的HPLC指纹图谱研究
TITLE:
摘要: 目的:建立红丝线药材的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为ODS-C18,流动相为甲醇-水(梯度洗脱),流速为1.0 ml/min,检测波长为370 nm,柱温为30 ℃,进样量为20 μl。以2号色谱峰为参照峰,测定11 批样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行共有峰指认和相似度评价。结果:11批药材样品HPLC图谱有10个共有峰,相似度均≥0.965。经验证,11批样品HPLC图谱与对照图谱具有较好的一致性。结论:该试验所建立的HPLC指纹图谱专属性强、结果稳定,可作为红丝线药材质量评价的主要依据之一。
ABSTRACT: OBJECTIVE: To establish the HPLC fingerprints of Lycianthes biflora. METHODS: HPLC was performed on the column of Dima ODS-C18 with mobile phase of methanol-water (gradient elution) at a flow rate of 1.0 ml/min, the detection wavelength was 370 nm, column temperature was 30 ℃,and injection volume was 20 μl. Using chromatographic peak 2, the HPLC chromatograms of 11 batches were determined, and common peak identification and similarity evaluation were conducted by using Fingerprint Similarity Evaluation System of Traditional Chinese Medicine (version A in 2004). RESULTS: There were 10 common peaks in the HPLC chromatograms of 11 batches, the similarity was no lower than 0.965. According to the verification, the HPLC chromatograms of 11 batches and reference patterns had a good consistency. CONCLUSIONS: The established fingerprint is specific, stable, and can be used for one of the main basis of quality evaluation of L. biflora.
期刊: 2016年第27卷第36期
作者: 曹庆玺,邓洁媚,姬生国,王东
AUTHORS: CAO Qingxi,DENG Jiemei,JI Shengguo,WANG Dong
关键字: 红丝线;指纹图谱;高效液相色谱法;质量评价
KEYWORDS: Lycianthes biflora; Fingerprint; HPLC; Quality evaluation
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