市售栀子药材的质量标准研究
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篇名: 市售栀子药材的质量标准研究
TITLE:
摘要: 目的:建立市售栀子药材的质量标准。方法:测定药材的水分、总灰分和浸出物。采用高效液相色谱法测定药材中栀子苷、芦丁、西红花苷Ⅰ和西红花苷Ⅱ的含量:色谱柱为Waters Xbridge -C18,流动相为乙腈-0.2%磷酸(梯度洗脱,栀子苷、芦丁)、甲醇-水(45 ∶ 55,V/V,西红花苷Ⅰ、西红花苷Ⅱ),流速为1.0 ml/min,检测波长为256 nm(栀子苷、芦丁)、440 nm(西红花苷Ⅰ、西红花苷Ⅱ),柱温为30 ℃,进样量为10 μl(栀子苷、芦丁)、5 μl(西红花苷Ⅰ、西红花苷Ⅱ)。结果:栀子药材的水分、总灰分和浸出物分别为5.8%~8.4%、3.7%~5.9%和29.5%~37.9%。栀子苷、芦丁、西红花苷Ⅰ和西红花苷Ⅱ检测质量浓度线性范围分别为162.08~1 620.84 μg/ml(r=0.999 9)、2.07~20.72 μg/ml(r=0.999 9)、8.04~80.41 μg/ml(r=0.999 9)、1.05~10.53 μg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;加样回收率分别为98.65%~101.43%(RSD=1.09%,n=6)、97.97%~101.83%(RSD=1.39%,n=6)、97.97%~101.30%(RSD=1.36%,n=6)、98.49%~103.04%(RSD=1.84%,n=6)。结论:该研究所建标准可用于市售栀子药材的质量控制。
ABSTRACT: OBJECTIVE: To establish the quality standard for Gardenia jasminoides. METHODS: The moisture, total ash and extract were determined. HPLC was used for contents determination of geniposide, rutin, crocinⅠand crocinⅡ: the column was Waters Xbridge-C18 with mobile phase of acetonitrile-0.2% phosphoric acid (gradient elution, gardenia and rutin), methanol-water (45 ∶ 55,V/V, crocinⅠ, crocinⅡ) at a flow rate of 1.0 ml/min; detection wavelength was 256 nm for  gardenia and rutin, 440 nm for crocinⅠ, crocinⅡ; column temperature was 30 ℃; injection volume was 10 μl for  gardenia and rutin, 5 μl for crocinⅠ, crocinⅡ. RESULTS: The moisture total ash and ethanol-soluble extract of G. jasminoides were 5.8%-8.4%,3.7%-5.9% and 29.5%-37.9%, respectively. The linear range was 162.08-1 620.84 μg/ml for geniposide (r=0.999 9), 2.07-20.72 μg/ml for rutin (r=0.999 9), 8.04-80.41 μg/ml for crocinⅠ(r=0.999 9) and 1.05-10.53 μg/ml for crocinⅡ(r=0.999 9); RSDs of precision, stability and reproducibility test were lower than 2%; recoveries were 99.33%-101.43% (RSD=1.09%,n=6), 97.97%-101.83% (RSD=1.39%,n=6), 97.97%-101.30%(RSD=1.36%,n=6) and 98.65%-103.04% (RSD=1.84%,n=6). CONCLUSIONS: The established standard can be used for the quality control of G. jasminoides commercially available.
期刊: 2016年第27卷第27期
作者: 王晓,黄南
AUTHORS: WANG Xiao,HUANG Nan
关键字: 栀子;质量控制;栀子苷;芦丁;西红花苷Ⅰ;西红花苷Ⅱ;高效液相色谱法
KEYWORDS: Gardenia jasminoides; Quality control; Geniposide; Rutin; CrocinⅠ; CrocinⅡ; HPLC
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