HPLC法测定栀制人参中人参皂苷Rg1、Re、Rb1和栀子苷的含量
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篇名: HPLC法测定栀制人参中人参皂苷Rg1、Re、Rb1和栀子苷的含量
TITLE:
摘要: 目的:建立测定栀制人参中人参皂苷Rg1、Re、Rb1和栀子苷含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent TC18,流动相为乙腈-0.1%磷酸(梯度洗脱,人参皂苷Rg1、Re、Rb1)、乙腈-水(13 ∶ 87,V/V,栀子苷),流速为1.0 ml/min,检测波长为203 nm(人参皂苷Rg1、Re、Rb1)、238 nm(栀子苷),柱温为40 ℃,进样量为20 μl。结果:人参皂苷Rg1、Re、Rb1和栀子苷检测进样量线性范围分别为0.418~3.762 μg(r=0.999 8)、0.270~2.430 μg(r=0.999 8)、0.398~3.582 μg(r=0.999 7)、0.606~3.030 μg(r=0.999 7);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.86%~101.47%(RSD=1.29%)、96.21%~100.11%(RSD=1.42%)、96.24%~100.48%(RSD=1.57%)、97.76%~102.44%(RSD=1.71%),n均为6。结论:该方法操作简便,精密度、稳定性、重复性好,可用于 栀制人参中人参皂苷Rg1、Re、Rb1和栀子苷含量的测定。
ABSTRACT: OBJECTIVE: To establish a method for the contents determination of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and geniposide in Panax ginseng processed by Gardenia jasminoides. METHODS: HPLC was performed on the column of Agilent TC18 with mobile phase of acetonitrile-0.1% phosphoric acid, (gradient elution, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1) and acetonitrile-water (13 ∶ 87,V/V, geniposide) at a flow rate of 1.0 ml/min, the detection wavelength was 203 nm for ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and 238 nm for geniposide, column temperature was 40 ℃, and injection volume was 20 μl. RESULTS: The linear range was 0.418-3.762 μg for ginsenoside Rg1(r=0.999 8), 0.270-2.430 μg for Re(r=0.999 8),0.398-3.582 μg for ginsenoside Rb1(r=0.999 7) and 0.606-3.030 μg for geniposide(r=0.999 7); RSDs of precision, stability and reproducibility tests were less than 2.0%;recoveries were 97.86%-101.47%(RSD=1.29%,n=6), 96.21%-100.11% (RSD=1.42%,n=6), 96.24%-100.48%(RSD=1.57%,n=6) and 97.76%-102.44% (RSD=1.71%,n=6). CONCLUSIONS: The method is simple with good precision, stability and reproducibility, and can be used for the contents determination of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and geniposide in P. ginseng processed by Gardenia jasminoides.
期刊: 2016年第27卷第24期
作者: 曲琰,贾天柱
AUTHORS: QU Yan1,JIA Tianzhu
关键字: 栀制人参;人参皂苷;栀子苷;含量;高效液相色谱法
KEYWORDS: Panax ginseng processed by Gardenia jasminoides; Ginsenoside; Geniposide; Content; HPLC
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