酸枣仁汤对老年失眠模型大鼠学习记忆能力及脑内神经递质含量的影响
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篇名: 酸枣仁汤对老年失眠模型大鼠学习记忆能力及脑内神经递质含量的影响
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摘要: 目的:研究酸枣仁汤对老年失眠模型大鼠学习记忆能力及脑内神经递质含量的影响。方法:将大鼠随机分为正常组、模型组、阳性组[艾司唑仑片,2×10-3 g/(kg·d)]和酸枣仁汤低、中、高剂量组[5、10、15 g/(kg·d)],每组10只。除正常组外,其余各组大鼠均于颈背部ih D-半乳糖+睡眠剥夺以复制老年失眠模型,造模6周后各给药组大鼠连续ig相应药物1周,正常组和模型组大鼠ig生理盐水。观察大鼠逃避潜伏期、原平台象限游泳时间百分比、穿过原平台位置的次数,测定大鼠脑组织中谷氨酸(Glu)、γ-氨基丁酸(GABA)和下丘脑中白细胞介素1β(IL-1β)、5-羟色胺(5-HT)含量。结果:与正常组比较,各组大鼠逃避潜伏期延长、原平台象限游泳时间百分比降低、穿过原平台位置的次数减少,脑组织中Glu、GABA含量增加,下丘脑中IL-1β、5-HT含量减少(P<0.05);与模型组比较,各给药组大鼠上述指标均得到明显改善,差异有统计学意义(P<0.05);与阳性组比较,酸枣仁汤中、高剂量组大鼠上述指标变化更为明显,差异有统计学意义(P<0.05)。结论:酸枣仁汤可明显增加老年失眠模型大鼠的学习记忆能力,且可降低脑内神经递质含量、减轻Glu、GABA参与的迟发性神经元损害。
ABSTRACT: OBJECTIVE: To study the effects of Suanzaoren decoction on learning and memory ability and brain neurotransmitters content of senile insomnia model rats. METHODS: Rats were randomly divided into normal group, model group, positive group [Estazolam tablet, 2×10-3 g/(kg·d)] and Suanzaoren decoction low-dose, middle-dose and high-dose groups [5, 10, 15      g/(kg·d)], with 10 rats in each group. Except for normal group, those groups were given D-galactose ih on the back+sleep deprivation to induce senile insomnia model. 6 weeks after modeling, treatment groups were given relevant medicine intragastrically for one week, and normal group and model group were given normal saline intragastrically. The escape latency, the percentage of swimming time and the time of original platform crossing were observed, and the contents of Glu and GABA in cerebral tissue were determined as well as the contents of IL-1β and 5-HT in hypothalamus. RESULTS: Compared with normal group, escape latency of rats prolonged and the contents of IL-1β and 5-HT in hypothalamus decreased, while the percentage of swimming time of original platform quadirant, the time of original platform crossing and the contents of Glu and GABA in cerebral tissue increased (P<0.05). Compared with model group, above index of treatment groups improved significantly, with statistical significance (P<0.05). Compared with positive group, the changes of above index were more significant in Suanzaoren decoction middle-dose and high-dose groups, with statistical significance (P<0.05). CONCLUSIONS: Suanzaoren decoction could markedly enhance the learning and memory ability of senile insomnia model rats, and reduce neurotransmitters content and relieve the delayed neuronal damage which led by Glu and GABA.
期刊: 2016年第27卷第22期
作者: 郭杰,尹晓刚
AUTHORS: GUO Jie,YIN Xiaogang
关键字: 酸枣仁汤;老年失眠模型大鼠;学习记忆能力;神经递质
KEYWORDS: Suanzaoren decoction; Senile insomnia model rats; Learning and memory ability; Neurotransmitter
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