栀子金花丸HPLC指纹图谱及其与体外抗氧化活性的相关性分析
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篇名: 栀子金花丸HPLC指纹图谱及其与体外抗氧化活性的相关性分析
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摘要: 目的:建立栀子金花丸指纹图谱,并分析与其体外抗氧化活性之间的相关性,为栀子金花丸的质量控制提供依据。方法:采用高效液相色谱法(HPLC)。色谱柱为Sinochrom ODS-BP C18(200 mm×4.6 mm,5 μm),流动相为0.2%磷酸溶液(含3 mmol/L庚烷磺酸钠溶液)-乙腈(梯度洗脱),检测波长为254 nm,流速为0.8 ml/min,柱温为38 ℃,进样量为10 μl。采用国家药典委员会“中药色谱指纹图谱相似度评价系统软件”(2012.130723版)对12批栀子金花丸液相图谱进行相似度评价,以黄芩苷为参照峰,对各指纹共有峰进行归属。采用1,1-二苯基-2-三硝基苯肼自由基清除试验考察12批栀子金花丸的体外抗氧化活性,并对其指纹图谱与体外抗氧化活性之间的谱-效关系进行研究。结果:建立了栀子金花丸指纹图谱,12批次栀子金花丸指纹图谱与对照图谱相似度均在0.9以上(S1、S2、S3、S12除外);标记了30个共有峰,并全部归属到各成方药材中;体外抗氧化试验结果显示,不同批次栀子金花丸抗氧化能力存在差异;谱-效关系结果显示,13个共有峰与抗氧化活性呈正相关,17个共有峰呈负相关,在已知成分中发挥抗氧化活性的成分主要集中在金银花、黄芩和大黄3味药材中。结论:所建立的指纹图谱及其与抗氧化活性的谱-效关系可为该药的质量控制提供参考。
ABSTRACT: OBJECTIVE: To establish fingerprint of Zhizi jinhua pills (ZZJHW) and analyze the relationship of it with in vitro antioxidant activity, in order to provide the basis for the quality control of them. METHODS: HPLC method was adopted. The separation was performed on a Sinochrom ODS-BP C18 (200 mm×4.6 mm, 5 μm) column with mobile phase consisted of 0.2% acetic acid (containing 3 mmol/L sodium heptanesulfonate solution)-acetonitrile (gradient elution) at the detection wavelength of 254 nm and flow rate of  0.8 ml/min. The column temperature was controlled at 38 ℃, and injection volume was 10 μl. The “Chromatographic Fingerprint Similarity Evaluation System for TCM” (2012.130723 edition) issued by Chinese Pharmacopoeia Commission was used to evaluate the similarity of the 12 batches of ZZJHW using baicalin as reference peak so as to attribute the common peak of fingerprint. DPPH free radical scavenging assay was used to investigate the in vitro antioxidant activity of 12 batches of ZZJHW, and the relationship between its fingerprint and antioxidant activity was studied. RESULTS: The fingerprint of 12 batches of ZZJHW was established and the similarity between the fingerprint of ZZJHW with their reference fingerprint were all above 0.9 (except S1,S2,S3,S12). 30 common peaks were marked, all of which were assigned to the herbs. Antioxidant experiment result showed the differences in the antioxidant capacity among different batches of ZZJHW; spectrum effect relationship showed that 13 common peaks were positively related with oxidation activity and 17 common peaks negatively related with it; among known components, oxidation activity components were mainly from Lonicera japonica, Scutellaria baicalensis and Rheum palmatum. CONCLUSIONS: The spectrum effect relationship of established fingerprint with its antioxidant activity can provide reference for the quality control of ZZJHW.
期刊: 2016年第27卷第22期
作者: 陈帅,王慧竹,薛健飞,钟方丽,李玲丽
AUTHORS: CHEN Shuai,WANG Huizhu,XUE Jianfei,ZHONG Fangli,LI Lingli
关键字: 栀子金花丸;指纹图谱;高效液相色谱法;抗氧化
KEYWORDS: Zhizi jinhua pills; Fingerprint; HPLC; Antioxidation
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