HPLC法测定白芥子饮片中白芥子苷的含量
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篇名: HPLC法测定白芥子饮片中白芥子苷的含量
TITLE:
摘要: 目的:建立测定白芥子饮片中白芥子苷含量的方法。方法:采用高效液相色谱法。色谱柱为Sepax HP-C18,流动相为50%乙腈-水溶液(含5 mmol/L四正辛基溴化铵),流速为1.0 ml/min,检测波长为227 nm,柱温为30 ℃,进样量为10 μl。结果:白芥子苷的检测质量浓度线性范围为0.010 1~0.202 4 mg/ml(r=0.999 7);精密度、稳定性、重复性试验的RSD<2%;加样回收率为98.51%~104.62%,RSD=1.90%(n=9)。结论:该方法操作简便、结果准确可靠,可用于白芥子饮片中白芥子苷的含量测定。
ABSTRACT: OBJECTIVE: To establish a method for the content determination of sinalbin in Sinapis alba decoction piece. METHODS: HPLC was performed on the column of Sepax HP-C18 with mobile phase of 50% acetonitrile-water solution (containing 5 mmol/L tetraoctyl ammonium bromide) at a flow rate of 1.0 ml/min, detection wavelength was 227 nm, column temperature was 30 ℃, and the injection volume was 10 μl. RESULTS: The linear range of sinalbin was 0.010 1-0.202 4 mg/ml (r=0.999 7); RSDs of precision, stability and reproducibility tests were lower than 2%, recovery was 98.51%-104.62% (RSD=1.90%,n=9). CONCLUSIONS: The method is simple, accurate and reliable, and can be used for determination of sinalbin in Sinapis alba decoction piece.
期刊: 2016年第27卷第18期
作者: 徐敏,叶凡,赖先荣,杨艳
AUTHORS: XU Min,YE Fan,LAI Xianrong,YANG Yan
关键字: 白芥子;白芥子苷;高效液相色谱法;含量测定
KEYWORDS: Sinapis alba; Sinalbin; HPLC; Content determination
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