螺旋藻激酶的体外抗氧化作用研究
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篇名: 螺旋藻激酶的体外抗氧化作用研究
TITLE:
摘要: 目的:研究螺旋藻激酶(SPK)的体外抗氧化作用。方法:采用邻二氮菲-Fe2+氧化法、1,1-二苯基-2-三硝基苯肼(DPPH)法和邻苯三酚自氧化法,分别测定不同浓度SPK对羟基(OH-)自由基、DPPH自由基和对超氧阴离子(O2-)自由基的清除能力,并计算半数抑制浓度(IC50);采用普鲁士蓝反应法测定不同浓度SPK对Fe3+的还原能力(以吸光度值反映)。以上试验均以维生素C(VC)为阳性对照。结果:SPK对OH-自由基、DPPH自由基及O2-自由基的清除能力均呈一定的浓度依赖性,SPK对OH-自由基、DPPH自由基最大清除率分别86.82%、78.89%(IC50分别为54.31、0.636 g/L),比VC(64.77%、73.49%)高;SPK对O2-自由基最大清除率为78.31%(IC50为3.918 g/L),比VC(94.14%)低;在还原能力试验中,SPK使体系吸光度值增加,其最大吸光度值与VC相近,并呈明显浓度依赖性。结论:螺旋藻激酶具有明显的体外抗氧化活性。
ABSTRACT: OBJECTIVE: To study the anti-oxidative effects of spirulina kinase (SPK) in vitro. METHODS: The methods of phenanthroine-Fe2+ oxidation method, DPPH and auto-oxidation of pyrogallol were used to measure the effects of different concentrations of SPK on scavenging hydroxyl (OH-) free radical, DPPH free radical and superoxide anion (O2-) free radical; IC50 of SPK was calculated. Prussian blue reaction was used to determine total reducing ability (by absorbance) of different concentrations of SPK to Fe3+. Vitamin C (VC) was used as positive control in above trials. RESULTS: SPK could eliminate the OH- free radical, DPPH free radical  and O2- free radical in concentration-dependant manner, and the maximum elimination rate of SPK to OH- free radical and DPPH free radical were 86.82% and 78.98% (IC50 were 54.31, 0.636 g/L), which were higher than VC (64.77%, 73.49%). The maximum elimination rate of SPK to O2- free radical was 78.31% (IC50 was 3.918 g/L), which was lower than VC (94.14%). In reducing ability test, SPK improved absorbance in reducing ability test system, and maximum absorbance was similar to VC in concentration-dependant manner. CONCLUSIONS: SPK has obvious anti-oxidant activities in vitro.
期刊: 2016年第27卷第16期
作者: 黄媛恒,庞辉,王慧杰,黎钦蓉,纪舒妤,黄为然,凌家杰,李映新
AUTHORS: HUANG Yuanheng,PANG Hui,WANG Huijie,LI Qinrong,JI Shuyu,HUANG Weiran,LIN Jiajie,LI Ying- xin
关键字: 螺旋藻激酶;体外;抗氧化;自由基
KEYWORDS: Spirulina kinase; in vitro; Anti-oxidant; Free radicals
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