沉香叶不同提取部位的体外抗氧化作用研究
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篇名: 沉香叶不同提取部位的体外抗氧化作用研究
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摘要: 目的:考察沉香叶不同提取部位的体外抗氧化作用。方法:采用回流提取法依次用石油醚、乙酸乙酯、无水乙醇、水对沉香叶进行提取,得到相应部位提取物(分别记为AGP、AGE、AGA、AGW部位),考察各部位对2,2′ -联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS+)自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基、超氧阴离子(O2-)自由基的清除能力以及对铜离子(Cu2+)的还原能力,并计算半数抑制浓度(IC50)。以上试验均以维生素C(VC)为阳性对照。结果:各部位对上述自由基的清除作用及对Cu2+的还原作用均呈浓度依赖性,其中AGA、AGW部位对ABTS+自由基的最大清除率分别为100%、81.07%(IC50分别为14.89、23.12 mg/L),且高于VC(88%),其余部位清除率均未超过50%;AGA部位对DPPH自由基的最大清除率为67.74%(IC50为47.96 mg/L),  但不及VC(91.44%),其余部位清除率较弱,均低于40%;AGA、AGW部位对O2-自由基的最大清除率分别为67.79%、61.79%(IC50分别为393.01、421.04 mg/L),但不及VC(88%),其余部位清除率未超过33%;AGP、AGE、AGA、AGW部位对Cu2+最大还原百分率均超过50%(IC50分别为55.99、40.27、25.44、31.06 mg/L),但均不及VC(100%)。结论:沉香叶各部位提取物均有体外抗氧化活性,其中以无水乙醇部位抗氧化能力最强。
ABSTRACT: OBJECTIVE: To investigate antioxidant effects of different extract parts from Aquilaria sinensis leaves. METHODS: A. sinensis leaves were extracted by reflux extraction with petroleum ether, ethyl acetate, absolutely ethanol, water to obtain corresponding parts (AGP, AGE, AGA and AGW parts). The ability of different extracts eliminating ABTS+ free radical, DPPH free radical and O2- free radical and reducing Cu2+ were investigated. IC50 was also calculated. Vitamin C (VC) was used as positive control in above tests. RESULTS: Those parts eliminated above free radicals and reduced Cu2+ in concentration-dependant manner, among which maximal elimination rate of AGA and AGW parts to ABTS+ free radical were 100% and 81.07% (IC50=14.89, 23.12 mg/L) and higher than that of VC (88%); those of other parts were less than 50%. The maximal elimination rate of AGA parts to DPPH free radical was 67.74% (IC50=47.96 mg/L), but still lower than that of VC (91.44%); the elimination rates of other parts were all weak and lower than 40%. The maximal elimination rates of AGA and AGW parts to O2- free radical were 67.79%  and 61.79% (IC50=393.01, 421.04 mg/L), but still lower than that of VC (88%); the elimination rates of other parts were all lower than 33%. The maximal reduction percentage of AGP, AGE, AGA and AGW parts to Cu2+ were all higher than 50% (IC50=55.99, 40.27, 25.44, 31.06 mg/L, respectively), but still lower than that of VC (100%). CONCLUSIONS: Different extract parts from A. sinensis leaves have antioxidant activity in vitro, and that of AGA is the strongest.
期刊: 2016年第27卷第16期
作者: 邓幸运,周绿颖,李远彬,易智彪,赖小平,王羚郦
AUTHORS: DENG Xingyun,ZHOU Lüeying,LI Yuanbin,YI Zhibiao,LAI Xiaoping,WANG Lingli
关键字: 沉香叶;提取物;抗氧化能力;清除作用;体外
KEYWORDS: Aquilaria sinensis leaves; Extracts; Antioxidant ability; Scavenging effect; in vitro
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