甘草多糖对CCl4诱导急性肝损伤模型小鼠的保护作用及其机制研究
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篇名: 甘草多糖对CCl4诱导急性肝损伤模型小鼠的保护作用及其机制研究
TITLE:
摘要: 目的:研究甘草多糖对急性肝损伤模型小鼠的保护作用及其可能的作用机制。方法:60只ICR小鼠随机分为正常对照(生理盐水)组、模型(生理盐水)组、联苯双酯(阳性药物,100 mg/kg)组和甘草多糖高、中、低剂量(400、200、100 mg/kg)组,ig给药,每天1次。15 d后除正常对照组外其余各组小鼠ip CCl4诱发急性肝损伤模型,测定肝脏指数,检测血清中天冬氨酸转氨酶(AST)、谷氨酸转氨酶(ALT)及肝脏组织中一氧化氮合酶(NOS)、一氧化氮(NO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)水平。结果:与正常对照组比较,模型组小鼠的肝脏指数,血清中AST、ALT及肝脏组织中NOS、NO、MDA水平升高,肝脏组织中SOD、GSH-Px水平降低(P<0.05),肝细胞肿胀、变性、坏死,出现明显炎性损伤。与模型组比较,甘草多糖各剂量组小鼠肝脏指数降低,其中高、中剂量组差异有统计学意义(P<0.05);甘草多糖各剂量组小鼠血清中AST、ALT水平及肝脏组织中NOS、NO、MDA水平降低,肝脏组织中SOD和GSH-Px水平升高(P<0.05),肝细胞肿胀、变性、坏死程度减轻,病理损伤得到改善。结论:甘草多糖对CCl4诱导的小鼠急性肝损伤具有一定的保护作用;其机制可能与甘草多糖抗氧化,降低肝脏组织中NOS、NO水平,减少自由基的产生有关。
ABSTRACT: OBJECTIVE: To study the protective effect of Glycyrrhiza uralensis polysaccharide (GUP) on CCl4 induced acute liver injury in mice and its mechanism. METHODS: 60 ICR mice were randomly divided into normal control group (normal saline), model group (normal saline), bifendate group (positive drug, 100 mg/kg) and GUP high-dose, medium-dose and low-dose groups (400, 200 and 100 mg/kg). They were given relevant medicine intragastrically once a day. 15 d later, acute liver injury model was induced by intraperitoneal injection of CCl4 in those groups except for normal control group, and liver index was determined. The levels of AST and ALT in serum, the NOS, NO, SOD, GSH-Px and MDA levels in liver tissues were detected. RESULTS: Compared with normal control group, the liver index, the levels of AST and ALT in serum and NOS, NO and MDA in liver tissue increased in model group, while SOD and GSH-Px levels in liver tissue decreased (P<0.05); liver cells became swelling, degeneration and necrosis, showed obvious inflammatory injury. Compared with model group, the liver index of GUP groups decreased, there was statistical significance in GUP high-dose medium-dose groups (P<0.05); the levels of AST and ALT in serum and NOS, NO and MDA in liver tissue of mice decreased in GUP groups, while the level of SOD and GSH-Px in liver tissue increased (P<0.05); liver cellular swelling, degeneration and necrosis relieved, and pathological injury had been improved. CONCLUSIONS: GUP has a certain protective effect on CCl4-induced acute liver injury in mice and its protective effect may be related to antioxidation, decrease of NOS and NO levels, and reduction of the production of free radicals.
期刊: 2016年第27卷第10期
作者: 陈冬雪,陈亮
AUTHORS: CHEN Dongxue,CHEN Liang
关键字: 甘草多糖;急性肝损伤;抗氧化;自由基;四氯化碳;小鼠
KEYWORDS: Glycyrrhiza uralensis polysaccharide; Acute liver injury; Antioxidation; Free radicals; CCl4; Mice
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