山茱萸多糖对血管性痴呆大鼠学习记忆能力的影响及其机制 研究
x

请在关注微信后,向客服人员索取文件

篇名: 山茱萸多糖对血管性痴呆大鼠学习记忆能力的影响及其机制 研究
TITLE:
摘要: 目的:研究山茱萸多糖对血管性痴呆(VD)模型大鼠学习记忆能力的影响及其机制。方法:将30只大鼠随机均分为假手术(生理盐水)组、模型(生理盐水)组和山茱萸多糖(0.28 g/kg)组。除假手术组外,其余各组大鼠均复制VD模型,并同时ig相应药物,每天1次。4周后,测定各组大鼠跨越原平台位置次数、水迷宫平均逃避潜伏期、平台滞留时间、平均游泳路程,测定各组大鼠海马组织中脑源性神经生长因子(BDNF)、凋亡相关基因Bcl-2 mRNA及其蛋白的表达。结果:与假手术组比较,模型组大鼠的学习记忆能力下降,表现为跨越原平台位置次数减少、水迷宫平均逃避潜伏期延长、平台滞留时间缩短、平均游泳路程变长(P<0.05),海马组织中BDNF、Bcl-2 mRNA及其蛋白表达明显减弱(P<0.05);与模型组比较,山茱萸多糖组大鼠的学习记忆能力增强,表现为跨越原平台位置次数增加、水迷宫平均逃避潜伏期缩短、平台滞留时间延长、平均游泳路程变短(P<0.05),海马组织中BDNF、Bcl-2 mRNA及其蛋白表达明显增强(P<0.05)。结论:山茱萸多糖能够改善VD大鼠的学习记忆能力,其机制可能与上调海马组织中BDNF、Bcl-2的表达有关。
ABSTRACT: OBJECTIVE: To study the effects of Cornus officinalis polysaccharide on learning and memory ability of vascular dementia (VD) model rats and its mechanism. METHODS: 30 rats were randomly divided into sham operation (normal saline) group, model (normal saline) group and C. officinalis polysaccharide (0.28 g/kg) group. Except sham operation group, VD model was induced in other groups and given relevant medicine intragastrically once a day. After 4 weeks, the frequency of leaping over original platform position, water maze mean escape latencies, platform retention time and average swimming distance were detected, and the mRNA and protein expression of brain-derived neurotrophic factor (BDNF) and apoptosis related gene Bcl-2 in hippocampal tissue of rats were determined. RESULTS: Compared with sham operation group, learning and memory ability of rats in model group weakened, manifesting as the frequency of leaping over original platform position decreased, water maze mean escape latencies prolonged, platform retention time shortened, average swimming distance extended (P<0.05); mRNA and protein expression of BDNF and Bcl-2 in hippocampus decreased significantly (P<0.05). Compared with model group, learning and memory ability of rats in C. officinalis polysaccharide group increased, manifesting as the frequency of leaping over original platform position increased, water maze mean escape latencies shortened, platform retention time prolonged, average swimming distance shortened (P<0.05); mRNA and protein expression of BDNF and Bcl-2 in hippocampus was enhanced significantly (P<0.05). CONCLUSIONS: C. officinalis polysaccharide can improve the learning and memory ability of VD rats, and its mechanism may be associated with the expression up-regulation of BDNF and Bcl-2 in hippocampus.
期刊: 2016年第27卷第7期
作者: 李永格,刘庆春
AUTHORS: LI Yongge,LIU Qingchun
关键字: 山茱萸多糖;脑源性神经生长因子;凋亡相关基因;血管性痴呆;学习记忆;大鼠
KEYWORDS: Cornus officinalis polysaccharide; Brain-derived neurotrophic factor; Apoptosis related gene; Vascular dementia; Learning and memory; Rat
阅读数: 281 次
本月下载数: 3 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!