黄芩苷对人骨肉瘤MG63细胞凋亡及MMPs表达的影响
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篇名: 黄芩苷对人骨肉瘤MG63细胞凋亡及MMPs表达的影响
TITLE:
摘要:

目的:研究黄芩苷对人骨肉瘤MG63 细胞凋亡及基质金属蛋白酶(MMPs)表达的影响。方法:以0(空白对照)、5、10、20、

40、80、160、320 μg/ml 黄芩苷处理MG63 细胞24 h 后,检测细胞存活率和细胞中MMP-2、MMP-9 的表达量,计算半数抑制浓度

(IC50),观察细胞凋亡情况。结果:与空白对照比较,黄芩苷处理MG63 细胞后细胞存活率降低,凋亡细胞数量增加,细胞中

MMP-2 和MMP-9 表达量降低,并以320 μg/ml 黄芩苷处理时细胞中MMP-2 和MMP-9 表达量下降具有统计学意义(P<0.05);IC50

为(40.21±9.20)μg/ml,各作用效应均呈浓度依赖性。结论:黄芩苷可抑制MG63 细胞增殖,诱导其凋亡,较高质量浓度时可抑制

细胞中MMP-2、MMP-9 的表达。

ABSTRACT:

OBJECTIVE:To study the effects of baicalin on human osteosarcoma MG63 cells apoptosis and the expression of

MMPs. METHODS:Treated with 0(blank control),5,10,20,40,80,160,320 μg/ml baicalin for 24 h,the survival rate,the

expression amount of MMP-2 and MMP-9 were detected,and IC50 was calculated. Cell apoptosis was observed. RESULTS:Compared

with blank control group,after treated with baicalin,survival rate of MG63 cells decreased,while apoptotic amount increased,

the expression amount of MMP-2 and MMP-9 decreased;there was statistical significance in the expression amount decrease

of MMP-2 and MMP-9 in MG63 cells after treated with 320 μg/ml baicalin(P<0.05);IC50 was(40.21±9.20)μg/ml,all

responses were in concentration-dependent manner. CONCLUSIONS:Baicalin can inhibit the proliferation of MG63 cells,induce

cell apoptosis,and inhibit the expression of MMP-2 and MMP-9 in cells under high concentration.

期刊: 2016年第27卷第4期
作者: 沈志华,郭菁菁
AUTHORS: SHEN Zhihua,GUO Jingjing
关键字: 黄芩苷;人骨肉瘤MG63 细胞;基质金属蛋白酶;细胞凋亡
KEYWORDS: Baicalin;Human osteosarcoma MG63 cells;Matrix metalloproteinase;Cell apoptosis
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