HPLC法测定注射用硫酸长春新碱的含量
x

请在关注微信后,向客服人员索取文件

篇名: HPLC法测定注射用硫酸长春新碱的含量
TITLE:
摘要:

目的:建立测定注射用硫酸长春新碱含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent HC-C8,流动相为

1.67%二乙胺水溶液(用磷酸调pH 至7.5)-甲醇(38 ∶ 62,V/V),流速为1.0 ml/min,检测波长为297 nm,柱温为35 ℃,进样量为

10 μl。结果:硫酸长春新碱的检测质量浓度线性范围为0.032~3.2 mg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD<1%;

平均回收率99.3%~101.7%,RSD=0.6%(n=9)。结论:该方法准确、可靠、灵敏度高,可用于测定注射用硫酸长春新碱的含量。

ABSTRACT:

OBJECTIVE:To establish a method for the content determination of Vincristine sulfate for injection. METHODS:

HPLC was performed on the column of Agilent HC-C8 with mobile phase of 1.67% Diethylamine aqueous solution(adjusted to pH

7.5 with phosphoric acid)-methanol(38 ∶ 62,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 297 nm,the column

temperature was 35 ℃,and the injection volume was 10 μl. RESULTS:The linear range of vincristine sulfate was 0.032-3.2 mg/ml

(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 99.3%-101.7%(RSD=

0.6%,n=9). CONCLUSIONS:The method is accurate,reliable and sensitive,and can be used for the content determination of

Vincristine sulfate for injection.

期刊: 2016年第27卷第3期
作者: 朱荣
AUTHORS: ZHU Rong
关键字: 高效液相色谱法;注射用硫酸长春新碱;含量
KEYWORDS: HPLC;Vincristine sulfate for injection;Content
阅读数: 342 次
本月下载数: 7 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!