HPLC 法同时测定儿泻停糖浆中黄芩苷、盐酸小檗碱、葛根素的
x

请在关注微信后,向客服人员索取文件

篇名: HPLC 法同时测定儿泻停糖浆中黄芩苷、盐酸小檗碱、葛根素的
TITLE:
摘要:

目的:建立同时测定儿泻停糖浆中葛根素、盐酸小檗碱、黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为

Kromasil C18,流动相为甲醇-0.2%磷酸水溶液(梯度洗脱),流速为1.0 ml/min,柱温为30 ℃,检测波长为275 nm,进样量为10 μl。

结果:黄芩苷、盐酸小檗碱、葛根素的检测质量浓度线性范围分别为10.43~83.44、10.71~85.68、12.58~100.64 μg/ml;精密度、稳

定性、重复性试验的RSD<2%;加样回收率分别为98.25%~100.87%、99.41%~101.40%、98.13%~99.76%,RSD 分别为

0.92%、0.68%、0.57%(n=9)。结论:该方法操作简便,结果可靠,可用于儿泻停糖浆的质量控制。

ABSTRACT:

OBJECTIVE:To establish a method for simultaneous determination of puerarin,berberine hydrochloride and baicalin

in Erxieting syrup. METHODS:HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2%

Phosphoric acid solution(gradient elution)at flow rate of 1.0 ml/min,column temperature was 30 ℃,detection wavelength was

275 nm,and the volume injection was 10 μl. RESULTS:The linear was 10.43-83.44 μg/ml for puerarin,10.71-85.68 μg/ml for berberine

hydrochloride and 12.58-100.64 μg/ml for baicalin(r≥0.999 3);RSDs of precision,stability and reproduciblity tests were

lower than 2%;recoveries were 98.25%-100.87%(RSD=0.92%,n=6),99.41%-101.40%(RSD=0.68%,n=6)and 98.13%-99.76%

(RSD=0.57%,n=6),respectively. CONCLUSIONS:The method is simple,reliable,can be used for the quality control of Erxieting

syrup.

期刊: 2016年第27卷第3期
作者: 周越,陈建伟
AUTHORS: ZHOU Yue,CHEN Jianwei
关键字: 儿泻停糖浆;黄芩苷;盐酸小檗碱;葛根素;高效液相色谱法
KEYWORDS: Erxieting syrup;Puerarin;Berberine hydrochloride;Baicalin;HPLC
阅读数: 205 次
本月下载数: 3 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!