柴葛感冒合剂的质量标准研究
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篇名: 柴葛感冒合剂的质量标准研究
TITLE:
摘要:

目的:建立柴葛感冒合剂的质量标准。方法:采用薄层色谱(TLC)法对制剂中黄芩、白芍进行定性鉴别。采用高效液相

色谱法测定制剂中葛根素的含量:色谱柱为Zorbax Eclipse XDB-C18,流动相为甲醇-水(25 ∶75,V/V),流速为1.0 ml/min,检测波长

为250 nm,柱温为25 ℃,进样量为5 μl。结果:TLC图斑点清晰,分离度好。葛根素检测质量浓度线性范围为18~198 μg/ml(r=

0.999 9);精密度、稳定性、重复性试验的RSD<2%;加样回收率为99.04%~99.75%,RSD=0.28%(n=6)。结论:所建标准可用

于柴葛感冒合剂的质量控制。

ABSTRACT:

OBJECTIVE:To establish the quality standard for Chaige ganmao mixture. METHODS:TLC was conducted to identify

the Scutellaria baicalensis and Paeonia lactiflora;HPLC was conducted to determine the content of puerarin. The column was

Zorbax Eclipse XDB-C18 with mobile phase of methanol-water(25 ∶ 75,V/V)at flow rate of 1.0 ml/min,detection wavelength was

250 nm,and column temperature was 25 ℃,and the injection volume was 5 μl. RESULTS:TLC showed clear spots and good separation.

The linear range of puerarin was 18-198 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower

than 2%,recovery was 99.04%-99.75%(RSD=0.28%,n=6). CONCLUSIONS:The standard can be used for the quality control

of Chaige ganmao mixture.

期刊: 2016年第27卷第3期
作者: 陈倩倩,孙洪胜
AUTHORS: CHEN Qianqian,SUN Hongsheng
关键字: 柴葛感冒合剂;质量标准;薄层色谱法;高效液相色谱法;黄芩;白芍;葛根素
KEYWORDS: Chaige ganmao mixture;Quality standard;TLC;HPLC;Scutellaria baicalensis;Paeonia lactiflora;Puerarin
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