藏药长梗金腰中2个活性成分的提取富集工艺研究
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篇名: 藏药长梗金腰中2个活性成分的提取富集工艺研究
TITLE: Study on extraction and enrichment technology of 2 active components in Tibetan medicine Chrysosplenium axillare
摘要: 目的 研究藏药长梗金腰中chrysosplenosideA(CA)和chrysosplenosideI(CI)的提取富集工艺。方法采用高效液相色谱法测定CA、CI的含量。采用正交实验,以CA和CI的总转移率为评价指标,乙醇体积分数、浸提温度、浸提次数及料液比为考察因素,优化长梗金腰中CA、CI的提取工艺,并进行验证。以D101型大孔吸附树脂为吸附剂,CA和CI的总含量为评价指标,杂质和目标成分的洗脱剂体积分数及用量为考察因素,优化CA和CI的富集工艺,并进行验证。结果长梗金腰中CA、CI的最优提取工艺为:将长梗金腰药材粉末以8倍量的50%乙醇在室温下一次性超声提取45min。验证结果显示,长梗金腰中CA和CI的平均总转移率为95.43%,RSD为1.02%(n=3)。最优富集工艺为:将上柱溶液加入D101型大孔吸附树脂柱,静置1h;用4BV(柱床体积)20%乙醇洗脱杂质,再用4BV50%乙醇洗脱CA和CI。验证结果显示,长梗金腰中CA和CI的平均总含量为322.7mg/g,RSD为1.05%(n=3),平均富集倍数为11.61倍。结论本研究成功优化了长梗金腰中CA和CI的提取富集工艺,可为CA和CI的药物开发利用提供参考。
ABSTRACT: OBJECTIVE To study the extraction and enrichment technology of chrysosplenides A (CA) and I (CI) in Tibetan medicine Chrysosplenium axillare. METHODS HPLC method was used to determine the contents of CA and CI. The orthogonal experiment was used to optimize the extraction technology of CA and CI in C. axillare using total transfer rate of CA and CI as evaluation indexes, with volume fraction of ethanol, extraction temperature, extraction times and solid-liquid ratio as factors. The validation test was also performed. The enrichment technology of CA and CI in C. axillare was optimized using D101 macroporous adsorption resin as adsorbent, total contents of CA and CI as evaluation indexes, with the volume fraction and dosage of eluent for impurities and target components. The validation test was also performed. RESULTS The optimum extraction conditions of CA and CI from C. axillare were as follows: the medicinal powder of C. axillare was extracted by ultrasound at room temperature for 45 min at one time with 8 times of 50% ethanol. Results of validation tests showed that total transfer rate of CA and CI in C. axillare was 95.43% in average (RSD=1.02%, n=3). The optimal enrichment technology was as follows: the sample solution was added into D101 macroporous adsorption resin column and stood for 1 hour; the impurities were eluted with 20% ethanol 4 BV (column volume), and CA and CI were eluted with 50% ethanol 4 BV. The results of validation tests showed that total content of CA and CI was 322.7 mg/g in average (RSD=1.05%, n=3), with average enrichment multiple of 11.61 times. CONCLUSIONS The study has successfully optimized the extraction and enrichment technology of CA and CI from C. axillare, and can provide reference for the development and utilization of CA and CI.
期刊: 2023年第34卷第05期
作者: 黎运芬;陈思;尼珍;向佳美;慕泽泾;朱玉野;龚淑芳;任刚
AUTHORS: LI Yunfen,CHEN Si,Nizhen,XIANG Jiamei,MU Zejing,ZHU Yuye,GONG Shufang,REN Gang
关键字: 长梗金腰;提取;富集;工艺优化;chrysosplenoside A;chrysosplenoside I
KEYWORDS: Chrysosplenium axillare; extraction; enrich-
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