长叶纽子果根醇提物乙酸乙酯萃取部位的化学成分及体外抗炎活性研究
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篇名: 长叶纽子果根醇提物乙酸乙酯萃取部位的化学成分及体外抗炎活性研究
TITLE: Study on chemical constituents and in vitro anti-inflammatory activity of ethyl acetate extraction part from ethanol extract of the roots of Ardisia virens
摘要: 目的 对长叶纽子果根的化学成分进行分离鉴定,并初步评价单体化合物的体外抗炎活性。方法采用硅胶柱层析、ODS柱色谱等方法对长叶纽子果根70%乙醇提取物的乙酸乙酯萃取部位进行分离、纯化,根据理化性质和波谱数据对所得化合物进行结构鉴定。以脂多糖诱导建立RAW264.7细胞炎症模型,采用MTT法考察所得化合物的抗炎活性。结果从上述萃取部位中共分离出11个化合物,依次鉴定为西克拉敏皂苷元A(1)、α-菠甾醇(2)、(3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one(3)、(+)-angelicoidenol(4)、十八碳癸二烯酸-2,3-二羟丙基酯(5)、α-亚麻酸(6)、glycerolmonooleate(7)、5,5′-(4,7-hexadecadlene-1,16-diyl)bisresorcinol(8)、1(-3,5-dihydroxyphenyl)heptan-1-one(9)、5-heptylresorcinol(10)、5-n-nonylresorcinol(11)。体外抗炎结果显示,80、40、20、10、5μg/mL的化合物2、8~10均可不同程度地降低细胞存活率。结论化合物1~11均首次从该植物中分离得到,化合物8为新天然产物。化合物2、8~10具有一定的体外抗炎活性。
ABSTRACT: OBJECTIVE To isolate and ide ntify the chemical constituents of the root of Ardisia virens and preliminarily evaluate the in vitro anti-inflammatory activity of the compounds. METHODS The ethyl acetate extraction part from 70% ethanol extract of the root of A. virens were separated and purified by silica gel column chromatography ,ODS column chromatography , etc. The structures of the compounds were identified according to physical and chemical properties and spectral data. The inflammation model of RAW 264.7 cells was induced by lipopolysaccharide ,and anti-inflammatory activity of the compound was investigated by MTT assay. RESULTS A total of 11 compounds were isolated from the ethyl acetate extraction part ,and were identified as cyclamiretin A (1),α-spinasterol (2),(3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one (3), (+)-angelicoidenol(4),octadeca-dienoic acid- 2,3-dihydroxypropyl ester (5),α-linolenic acid (6),glycerol monooleate (7),5, 5′-(4,7-hexadecadlene-1,16-diyl)bisresorcinol(8),1-(3,5-dihydroxyphenyl)heptan-1-one(9),5-heptylresorcinol and (10) 5-n-nonylresorcinol(11). The in vitro anti-inflammatory results showed that 80,40,20,10,5 μg/mL of compounds 2,8,9 and 10 could reduce the cell survival rate in different degrees. CONCLUSIONS Compounds 1-11 are isolated from this plant for the first time,and compound 8 is a new natural product. Compound 2,8,9 and 10 show certain anti-inflammatory activity in vitro .
期刊: 2022年第33卷第17期
作者: 周永强,廖张蓉,叶洪波,殷鑫,魏鑫,周英
AUTHORS: ZHOU Yongqiang,LIAO Zhangrong,YE Hongbo,YIN Xin,WEI Xin,ZHOU Ying
关键字: 长叶纽子果根;醇提物;乙酸乙酯萃取部位;化学成分;抗炎活性
KEYWORDS: roots of Ardisia virens ;ethanol extract ;ethyl acetate extraction part ;chemical constituents ;anti-inflammatory activity
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