雪菊抗抑郁有效部位的筛选及其作用机制研究
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篇名: 雪菊抗抑郁有效部位的筛选及其作用机制研究
TITLE: Screening of effective anti-depressant part from Coreopsis tinctoria and study on its mechanism
摘要: 目的 筛选雪菊的抗抑郁有效部位,并对其作用机制进行研究。方法采用悬尾实验和强迫游泳实验考察雪菊75%乙醇提取物的30%、50%、70%、90%乙醇洗脱部位(CCTE)的抗抑郁作用。采用小鼠甩头实验、利血平拮抗实验、育亨宾毒性增强实验和体外单胺氧化酶(MAO)抑制实验,探究有效部位与5-羟色胺(5-HT)及去甲肾上腺素(NE)能神经相关的作用机制。结果雪菊75%乙醇提取物的50%、70%CCTE能显著缩短小鼠悬尾实验和强迫游泳实验的累计静止不动时间(P<0.05或P<0.01),增加小鼠的甩头次数(P<0.01),显著逆转利血平引起的眼睑下垂、肛温下降和运动不能(P<0.05或P<0.01),增加育亨宾毒性导致的小鼠死亡只数(P<0.01);雪菊中奥卡宁(CCT-6)、异奥卡宁(CCT-7)、花旗松素(CCT-8)对MAO的半数抑制浓度(IC50)分别为8.71、37.89、67.07µmol/L。结论雪菊75%乙醇提取物的50%、70%CCTE是雪菊抗抑郁的有效部位,其抗抑郁作用可能与增加5-HT浓度及活化NE能神经有关。CCT-6、CCT-7和CCT-8抑制MAO可能是雪菊抗抑郁的作用机制之一。
ABSTRACT: OBJECTIVE To screen the effective anti-depressant part from Coreopsis tinctoria and study its mechanism. METHODS The anti-depressant effects of 30%,50%,70% and 90% ethanol elution fractions from 75% ethanol extract of C. tinctoria(CCTE)were investigated by tail suspension test and forced swimming test. Mice head-drop test ,reserpine antagonistic test,yohimbine toxicity enhancement test and in vitro monoamine oxidase (MAO) inhibition test were used to explore the mechanism of the relationship between the effective parts and 5-hydroxytryptamine (5-HT) and norepinephrine (NE) nerves. RESULTS The 50% and 70%CCTE could significantly shorten the accumulative immobility time in tail suspension test and forced swimming test (P<0.05 or P<0.01),increase the number of head-shaking times (P<0.01),reverse the eyelid ptosis , hypothermia and immobility caused by hematopin (P<0.05 or P<0.01),and increase the number of dead mice caused by yohimbine toxicity (P<0.01). IC 50 of okanin (CCT-6),isookanin(CCT-7)and taxifolin (CCT-8)against MAO were 8.71,37.89 and 67.07 µmol/L,respectively. CONCLUSIONS The 50% and 70%CCTE are the effective anti-depressant parts of C. tinctoria . Its anti-depressant effect may be related to the reinforcement of 5-HT and the activation of NE nerves. The inhibition of CCT- 6, CCT-7 and CCT- 8 against MAO may be one of the anti-depressant mechanism of C. tinctoria .
期刊: 2022年第33卷第10期
作者: 马瑞,李爽,王祯,高娟娟,谢艾迪,韩海霞
AUTHORS: MA Rui,LI Shuang ,WANG Zhen,GAO Juanjuan ,XIE Aidi,HAN Haixia
关键字: 雪菊;抗抑郁;有效部位;单胺类神经递质;5-羟色胺;去甲肾上腺素能神经
KEYWORDS: Coreopsis tinctoria ; anti-depressant; effective part ; monoamine neurotransmitter ; 5-hydroxytryptamine;
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