猪牙皂皂苷对痰瘀互结型缺血性中风模型大鼠的保护作用及机制研究
x

请在关注微信后,向客服人员索取文件

篇名: 猪牙皂皂苷对痰瘀互结型缺血性中风模型大鼠的保护作用及机制研究
TITLE: Protective effect and mechanism of saponins from Gleditsia sinensis on ischemic stroke with phlegm and blood stasis model rats
摘要: 目的 研究猪牙皂皂苷对痰瘀互结型缺血性中风(ISPBS)模型大鼠的保护作用,并探究其作用机制。方法将119只大鼠随机分为正常组(生理盐水)、假手术组(生理盐水)、模型组(生理盐水)、尼莫地平组(阳性对照组,5mg/kg)和猪牙皂皂苷低、中、高剂量组(3.21、6.42、12.84mg/kg),每组17只。除正常组外,其余各组大鼠均采用高脂饲料饲养+线栓法复制ISPBS模型。测定或观察大鼠神经功能评分、脑组织含水量、脑组织病理形态学变化和血清中血液流变学指标[全血黏度、红细胞聚集指数、卡松黏度]、血脂四项[三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]、炎症因子[白细胞介素1β(IL-1β)、IL-10、肿瘤坏死因子α(TNF-α)]及脑组织中氧化应激指标[丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)]变化情况,并测定脑组织中B淋巴细胞瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)和半胱氨酸蛋白酶3(caspase-3)蛋白表达情况。结果与正常组比较,模型组大鼠神经功能评分、5项血清指标(TC、TG、LDL-C、TNF-α、IL-1β)、血液流变学指标和脑组织中MDA、NO含量以及Bax、caspase-3蛋白表达水平均显著升高(P<0.01),血清中HDL-C、IL-10水平和脑组织中SOD活性、Bcl-2蛋白表达水平均显著降低(P<0.01),脑组织出现核固缩、胞膜碎裂等明显病变。与模型组比较,各给药组上述指标均不同程度改善,其中猪牙皂皂苷高剂量组大鼠的上述指标变化差异均有统计学意义(P<0.01)。结论猪牙皂皂苷对ISPBS模型大鼠具有较好的保护作用,其机制可能与减少氧化损伤、减少促炎介质的产生和抑制神经元凋亡有关。
ABSTRACT: OB JECTIVE To study the protective effects of saponins from Gleditsia sinensis on ischemic stroke with phlegm and blood stasis (ISPBS)model rats ,and to explore its mechanism. METHODS Totally 119 rats were randomly divided into normal group (normal saline ),sham operation group (normal saline ),model group (normal saline ),nimodipine group (positive control group ,5 mg/kg),G. sinensis saponin low-dose ,medium-dose and high-dose groups (3.21,6.42 and 12.84 mg/kg),with 17 rats in each group. Except for normal group ,other groups were all given high-fat diet+suture-occluded method to induce ISPBS model. The neurological function score ,water content of brain tissue ,pathological morphology of brain tissue ,the changes of hemorheology indexes (whole blood viscosity , erythrocyte aggregation index , Casson-viscosity), four items of blood lipid [triacylglycerol (TG),total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol(HDL-C)] and inflammatory factors in serum and oxidative stress indexes [malondialdehyde (MDA),nitric oxide (NO),superoxide dismutase (SOD)] in brain tissue were determined or observed in rats. The protein expressions of B lymphocytoma 2(Bcl-2),Bcl-2 associated X protein (Bax)and caspase- 3 in cerebral tissue were also detected. RESULTS Compared with normal group ,the score of nerve function ,5 kinds of serum indexes (TC,TG,LDL-C,TNF-α,IL-1β), hemorheology indexes ,the contents of MDA and NO and protein expressions of Bax and caspase- 3 in cerebral tissue were all increased significantly in model group (P<0.01). The levels of HDL-C and IL- 10 in serum ,SOD activity and protein expression of Bcl- 2 in cerebral tissue were decreased significantly (P<0.01),and obvious lesions such as nuclear pyknosis and cell membrane fragmentation occurred in brain tissue. Compared with model group ,above indexes of administration groups were improved to different extents ,among which there was statistical significance in above indexes of G. sinensis saponin high-dose group (P< 0.01). CONCLUSIONS Saponin from G. sinensis has a good protective effect on ISPBS model rats. Its mechanism may beassociated with reducing oxidative damage , reducing the production of pro-inflammatory mediators and resisting neuronal apoptosis.
期刊: 2022年第33卷第09期
作者: 董娜娜,陈晓兰,邓铋莉,万静,谢树才,胡娟,罗晨月,曹国琼
AUTHORS: DONG Nana,CHEN Xiaolan ,DENG Bili,WAN Jing,XIE Shucai ,HU Juan,LUO Chenyue ,CAO Guoqiong
关键字: 猪牙皂皂苷;痰瘀互结型缺血性中风;氧化损伤;机制
KEYWORDS: saponins from Gleditsia sinensis ;ischemic
阅读数: 25 次
本月下载数: 2 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!