虎杖一体化饮片与传统饮片的指纹图谱及体外抗氧化活性比较
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篇名: 虎杖一体化饮片与传统饮片的指纹图谱及体外抗氧化活性比较
TITLE: Comparison of the fingerprints and in vitro antioxidant activity of decoction pieces of Polygonum cuspidatum by integrated processing and traditional processing
摘要: 目的 比较虎杖产地加工与炮制一体化(简称“一体化”)制备的饮片与传统饮片的指纹图谱及体外抗氧化活性差异。方法根据一体化工艺和传统工艺分别制备10批虎杖一体化饮片和10批传统饮片。采用高效液相色谱法建立虎杖两种饮片的指纹图谱,并进行比较;检测虎杖两种饮片对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、ABTS自由基、超氧自由基、羟自由基的清除率以及对Fe3+的还原能力,比较这两种饮片的体外抗氧化活性。结果虎杖两种饮片共有11个共有峰,其中一体化饮片共有17个共有峰,传统饮片共有13个共有峰;虎杖一体化饮片中6号峰(虎杖苷)和15号峰(大黄素-8-O-β-D-葡萄糖苷)的峰高明显高于传统饮片,传统饮片中13号峰(白藜芦醇)、17号峰(大黄素)和19号峰(大黄素甲醚)的峰高明显高于一体化饮片。体外抗氧化活性结果显示,虎杖一体化饮片和传统饮片对DPPH自由基、ABTS自由基、超氧自由基和羟基自由基均具有清除能力,对Fe3+也均具有还原能力。结论虎杖一体化炮制工艺较传统炮制工艺可更好地保留虎杖的有效成分,且虎杖一体化饮片的体外抗氧化活性强于传统饮片。
ABSTRACT: OBJECTIVE To compare t he diff erences of the fingerprint and in vitro antioxidant activity between decoction pieces of Polygonum cuspidatum by integrated technology of habitat processing and processing (short for IPDP )and traditional processing decoction pieces (short for TPDP ). METHODS Ten batches of IPDP and ten batches of TPDP were prepared by integrated technology and traditional technology ,respectively. HPLC method was used to establish and compare the fingerprints of IPDP and TPDP. The scavenging rates of DPPH free radical ,ABTS free radical ,superoxide free radical and hydroxyl free radical and reducing activity of Fe 3+ were detected for IPDP and TPDP. In vitro antioxidant activities were compared between IPDP and TPDP. RESULTS There were 11 common peaks in the fingerprints of IPDP and TPDP ,among which 17 came from IPDP and 13 came from TPDP. The peak heights of peak 6(polydatin)and peak 15(emodin-8-O-β-D-glucoside)in IPDP were significantly higher than those in the TPDP ,and the peak heights of peak 13(resveratrol),peak 17(emodin)and peak 19(physcion)in the TPDP were significantly higher than those in the IPDP. The results of in vitro antioxidant test showed that IPDP and TPDP had a certain scavenging capacity on DPPH free radical ,ABTS free radical ,superoxide free radical and hydroxyl free radicals ,and also had a certain reducing capacity on Fe 3+. CONCLUSIONS The integrated processing technology of P. cuspidatum has a good retention effect on the glycosides in P. cuspidatum ,and the in vitro antioxidant activity of IPDP is stronger than that of TPDP.
期刊: 2022年第33卷第05期
作者: 简祈盼,朱映睿,郑宇锟,成焕波,涂济源,王光忠
AUTHORS: JIAN Qipan,ZHU Yingrui ,ZHENG Yukun ,CHENG Huanbo ,TU Jiyuan ,WANG Guangzhong
关键字: 虎杖;产地加工与炮制一体化;指纹图谱;抗氧化活性
KEYWORDS: Polygonum cuspidatum ;integrated technology of habitat processing and processing ;fingerprint;antioxidant activity
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