UHPLC法结合超滤法测定连钱草中3种成分的血浆蛋白结合率
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篇名: UHPLC法结合超滤法测定连钱草中3种成分的血浆蛋白结合率
TITLE: Determination of Plasma Protein Binding Rate of 3 Components from Glechoma longituba by UHPLC Combined with Ultrafiltration
摘要: 目的:建立测定连钱草中迷迭香酸、咖啡酸、绿原酸血浆蛋白结合率的方法。方法:采用超高效液相色谱法结合超滤法测定连钱草中迷迭香酸、咖啡酸、绿原酸在新西兰兔体内的血浆蛋白结合率。以PhenomenexLuna®C18为色谱柱,以乙腈(A)-0.1%甲酸溶液(B)为流动相进行梯度洗脱,流速为0.5mL/min,柱温为45℃,检测波长为327nm,进样量为3μL。结果:在低、中、高质量浓度下,迷迭香酸的血浆蛋白结合率分别为(97.78±1.67)%、(94.32±1.42)%、(95.12±1.51)%(n=3),咖啡酸的血浆蛋白结合率分别为(90.12±2.33)%、(89.53±1.98)%、(90.23±1.56)%(n=3),绿原酸的血浆蛋白结合率分别为(63.23±2.12)%、(67.87±1.06)%、(62.34±1.34)%(n=3)。结论:所建方法操作简单、分析时间较短,可用于测定连钱草中迷迭香酸、咖啡酸、绿原酸的血浆蛋白结合率。
ABSTRACT: OBJECTIVE:To establish a method for the determination of plasma protein binding rate of rosmarinic acid ,caffeic acid and chlorogenic acid from Glechoma longituba . METHODS :UHPLC method combined with ultrafiltration method was adopted to determine the plasma protein binding rate of rosmarinic acid ,caffeic acid and chlorogenic acid from G. longituba in the plasma of New Zealand rabbits. The determination was performed on a Phenomenex Luna ® C18 column with mobile phase consisted of acetonitrile (A)-0.1% formic acid solution (B)(gradient elution )at the flow rate of 0.5 mL/min. The column temperature was set at 45 ℃,and the detection wavelength was 327 nm. The sample size was 3 μL. RESULTS:At low ,medium and high concentrations,the plasma binding rates of rosmarinic acid were (97.78 ± 1.67)% ,(94.32 ± 1.42)% ,(95.12 ± 1.51)% , respectively(n=3);those of caffeic acid were (90.12±2.33)%,(89.53±1.98)%,(90.23±1.56)%,respectively(n=3);those of chlorogenic acid were (63.23 ± 2.12)% ,(67.87 ± 1.06)% ,(62.34 ± 1.34)% ,respectively (n=3). CONCLUSIONS : Established method is easy to operate and shorter time for analysis. It can be used to determine the plasma protein binding rate of rosmarinic acid ,caffeic acid and chlorogenic acid in G. longituba .
期刊: 2021年第32卷第22期
作者: 陈伟康,刘德鸿,熊明朋,袁惠,周国平
AUTHORS: CHEN Weikang ,LIU Dehong ,XIONG Mingpeng ,YUAN Hui,ZHOU Guoping
关键字: 连钱草;迷迭香酸;咖啡酸;绿原酸;血浆蛋白结合率;超高效液相色谱;超滤法;兔
KEYWORDS: Glechoma longituba ;Chlorogenic acid ;Caffeic acid ;Rosmarinic a cid;Plasma protein binding rate ;UHPLC;
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