延龄草总皂苷对佐剂性关节炎模型大鼠的改善作用及机制
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篇名: 延龄草总皂苷对佐剂性关节炎模型大鼠的改善作用及机制
TITLE: Improvement Effects of Trillium tschonoskii Total Saponins on Adjuvant-induced Arthritis Model Rats and Its Mechanism
摘要: 目的:研究延龄草总皂苷(TTM)对佐剂性关节炎模型大鼠的改善作用及机制。方法:将SD大鼠随机分为造模组(n=44)和空白组(n=6),造模组大鼠注射完全弗氏佐剂以复制佐剂性关节炎模型,空白组大鼠同法注射等体积生理盐水。将造模成功的大鼠分为模型组、雷公藤多苷片组(40mg/kg)和TTM低、中、高剂量组(50、100、200mg/kg),每组6只。各给药组大鼠灌胃相应药物,空白组和模型组大鼠灌胃生理盐水,每天1次,连续9d。末次灌胃后,测定大鼠体质量、足肿胀度(双侧),并进行关节炎性评分;测定大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6含量;观察大鼠踝关节病理形态学变化;检测大鼠膝关节组织中NOD样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、胱天蛋白酶1(caspase-1)的蛋白表达水平;检测大鼠踝关节滑膜组织中NLRP3的蛋白表达水平。结果:与空白组比较,模型组大鼠双侧足肿胀度,血清中TNF-α、IL-1β、IL-6含量,膝关节组织中NLRP3、ASC、caspase-1蛋白表达水平,踝关节滑膜组织中NLRP3蛋白表达水平均显著升高(P<0.05或P<0.01);体质量显著降低(P<0.05);滑膜细胞增生明显,可见滑膜组织充血、炎性细胞浸润等。与模型组比较,TTM各剂量组大鼠上述指标水平大部分均显著逆转(P<0.05或P<0.01);滑膜细胞增生、滑膜组织充血及软骨细胞破坏等病理改变均减轻,炎症细胞浸润减少。结论:TTM可改善大鼠类风湿性关节炎;其作用机制可能与抑制NLRP3/caspase-1信号通路的活性,降低炎症因子TNF-α、IL-1β、IL-6的表达有关。
ABSTRACT: OBJECTIVE:To stu dy the improvement effects o f Trillium tschonos kii total sapo nins(TTM)on adjuvant-induced arthritis model rats and its mechanism. METHODS :SD rats were randomly divided into model group (n=44)and blank group (n=6). Model group was given Complete Freund ’s adjuvant to induce the adjuvant-induced arthritis model ;blank group was given constant volume of normal saline with same method. Model group were divided into model group ,Tripterygium wilfordii polyglycoside tablets group (40 mg/kg),TTM low-dose ,medium-dose and high-dose groups (50,100,200 mg/kg),with 6 rats in each group. Administration groups were given relevant medicine intragastrically ;blank group and model group were given normal saline intragastrically ,once a day ,for consecutive 9 days. After last intragastric administration ,the body weight and foot swelling degree (bilateral)were detected ,and arthritis score was performed. The contents of tumor necrosis fator α(TNF-α), interleukin 1β(IL-1β)and IL- 6 were detected ;the pathomorphological changes of rat ankle were observed ;protein expression of NOD-like receptor protein 3 (NLRP3),apoptosis associated spot-like protein (ASC),caspase-1 were detected ;the protein expression of NLRP 3 in synovial tissue of ankle joint were also determined. RESULTS :Compared with blank group ,foot swelling degree,serum contents of TNF-α,IL-1β and IL-6,the protein expression of NLRP 3,ASC and caspase- 1 in knee tissue as well as the protein expression of NLRP 3 in synovial tissue of ankle joint were increased significantly in model group (P<0.05 or P< 0.01),while the body weight was decreased significantly (P<0.05). The obvious proliferation of synovial cells ,the congestion and inflammatory cell infiltration of synovial tissue were all observed. Compared with model group ,most of the above indexes were all reversed significantly in TTM groups (P<0.05 or P<0.01);the pathological changes such as synovial cell proliferation , congestion of synovial tissue and chondrocyte destruction were all relieved ,and inflammatory cell infiltration was alleviated.CONCLUSIONS:TTM can improve rheumatoid arthritis of rats, the mechanism of which may be associated with 用。E-mail:yulingling@ctgu.edu.cn inhibiting the activity of NLRP 3/caspase-1 signaling pathway and decreasing the expression of inflammatory factors TNF-α,IL-1β,IL-6.
期刊: 2021年第32卷第21期
作者: 谭晓茜,史伟文,蒋安蓉,柳蔚,李世刚,喻玲玲
AUTHORS: TAN Xiaoxi ,SHI Weiwen ,JIANG Anrong ,LIU Wei,LI Shigang ,YU Lingling
关键字: 延龄草总皂苷;关节炎;NOD样受体蛋白3;抗炎;大鼠
KEYWORDS: Trillium tschonoskii total saponins ;Arthritis;NOD-like receptor protein 3;Anti-inflammation;Rats
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