瑶药消瘤藤的质量标准研究
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篇名: 瑶药消瘤藤的质量标准研究
TITLE: Study on Quality Standard of Yao Medicine Pileostegia tomentella
摘要: 目的:建立瑶药消瘤藤的质量标准,为其质量控制提供参考。方法:采集10批广西产消瘤藤药材,观察其性状,并对其根、茎的横切面以及药材粉末进行显微鉴别;以7-羟基香豆素为指标成分建立该药材的薄层色谱(TLC)鉴别方法;测定该药材中水分、总灰分、酸不溶性灰分和醇溶性浸出物;采用高效液相色谱(HPLC)法同时测定该药材中茵芋苷、7-羟基香豆素和7-羟基-8-甲氧基香豆素的含量。结果:消瘤藤根和茎表面皱纹明显、质硬,气微、味微苦,横切面有分泌细胞和针晶细胞分布;药材粉末中有草酸钙针晶、草酸钙方晶、石细胞和淀粉粒。TLC结果显示,供试品色谱中,在与7-羟基香豆素对照品色谱相应的位置上,显相同颜色的荧光斑点。10批药材的水分测定结果为9.1%~11.8%,总灰分为3.6%~6.7%,酸不溶性灰分为0.10%~0.44%,醇溶性浸出物为8.9%~14.0%。茵芋苷、7-羟基香豆素和7-羟基-8-甲氧基香豆素的质量浓度分别在6.088~182.640、1.568~47.040、1.096~32.880μg/mL范围内与其对应色谱峰峰面积成良好的线性关系(r均不低于0.9997);平均加样回收率分别为99.47%、101.07%、99.89%(RSD均小于2%);精密度、稳定性(24h)、重复性、耐用性试验的RSD均小于2%。茵芋苷等3种待测成分的含量分别为1.337~9.534、0.348~2.236、0.083~1.088mg/g。结论:初步拟定消瘤藤中水分不得过12.0%、总灰分不得过7.0%、醇溶性浸出物不得少于8.0%、茵芋苷含量不得少于1.30mg/g、7-羟基香豆素含量不得少于0.30mg/g、7-羟基-8-甲氧基香豆素含量不得少于0.06mg/g。
ABSTRACT: OBJECTIVE:To establi sh the quality standard of Yao medicine Pileostegia tomentella,and to provide reference for its quality control. METHODS :Totally 10 batches of P. tomentella were collected from Guangxi area. The properties of P. tomentella were observed and microscopic identification was conducted for the transverse section of its stems ,roots and powder. TLC method was established by using 7-hydroxycoumarin as reference. The contents of water ,total ash ,acid-insoluble ash and alcohol-soluble extract in P. tomentella were determined. The contents of skimmin ,7-hydroxycoumarin and 7-hydroxy-8- methoxycoumarin in P. tomentella were determined by HPLC. RESULTS :The root and stem surface of P. tomentella possessed obvious wrinkles ,hard texture ,slight smell and bitter taste ,and there were secretory cells and needle crystal cells in the cross section. The medicinal powder contained calcium oxalate needle crystal ,calcium oxalate square crystal ,sclerotic cell and starch granules. The results of TLC showed that the spots of the same color were found in the corresponding positions of chromatograms of test samples and 7-hydroxycoumarin control. In the 10 batch of P. tomentella ,the contents of water were 9.1%-11.8%;those of total ash were 3.6%-6.7%;those of acid-insoluble ash were 0.10%-0.44%;those of alcohol-soluble extract were 8.9%-14.0%.The linear range of skimmin ,7-hydroxycoumarin and 7-hydroxy-8-methoxycoumarin were 6.088-182.640,1.568-47.040 and 1.096- 32.880 μg/mL(all r not less than 0.999 7). The average recoveries were 99.47%,101.07% and 99.89%(RSD were all less than 2%). RSDs of precision ,stability(24 h),reproducibility and durability tests were all lower than 2% . The contents of 3 components such as skimmin were 1.337-9.534,0.348-2.236, 0.083-1.088 mg/g,respectively. CONCLUSIONS :It is preli- 201705) minarily proposed that the content of water in P. tomentella is not more than 12.0% ;that of total ash is not more than 7.0%;that of alcohol-soluble extract is not less than 8.0%; E-mail:luguoshou@foxmail.com that of ski mmin is not less than 1.30 mg/g;that of 7-hydroxy- coumarin is not less than 0.30 mg/g;that of 7-hydroxy-8-methoxycoumarin is not less than 0.06 mg/g.
期刊: 2021年第32卷第21期
作者: 黄建猷,胡筱希,谭晓,屈信成,陆国寿,黄周锋
AUTHORS: HUANG Jianyou ,HU Xiaoxi ,TAN Xiao,QU Xincheng ,LU Guoshou ,HUANG Zhoufeng
关键字: 瑶药;消瘤藤;质量标准;薄层色谱法;高效液相色谱法;显微鉴别;性状鉴别;茵芋苷;7-羟基香豆素;7-羟基-8-甲氧基香豆素
KEYWORDS: Yao medicine ;Pileostegia tomentella;Quality standard ;TCL;HPLC;Microscopic identification ; Character
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