黄芪注射液中次生成分作用于白细胞减少症模型小鼠的代谢组学研究
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篇名: 黄芪注射液中次生成分作用于白细胞减少症模型小鼠的代谢组学研究
TITLE: Metabonomics Study on the Effects of Secondary Components of Astragalus membranaceus Injection on Leuko- penia Model Mice
摘要: 目的:研究黄芪注射液中次生成分作用于白细胞减少症模型小鼠的代谢组学。方法:将小鼠分为正常组、模型组、黄芪注射液组(0.004mL/g)及其次生成分低、中、高剂量组(0.004、0.008、0.016mL/g),每组7只。除正常组外,其余各组小鼠均腹腔注射环磷酰胺(80mg/kg)以复制白细胞减少症模型。造模成功后,各给药组小鼠腹腔注射相应药物,正常组和模型组小鼠腹腔注射等体积无菌注射用水,每天1次,连续7d。实验期间,每隔2d测定小鼠的体质量变化。末次给药后,采用动物血液分析仪检测小鼠的血常规指标[白细胞(WBC)、中性粒细胞(NE)、淋巴细胞(LY)、单核细胞(MO)计数],采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法,结合多元统计分析方法,对小鼠血清进行代谢组学分析。结果:与模型组比较,次生成分低剂量组小鼠体质量在给药第4、8天时均显著增加(P<0.05),次生成分各剂量组小鼠血清中WBC、NE、LY、MO计数均显著升高(P<0.05或P<0.01)。经代谢组学分析显示,黄芪注射液中次生成分可显著回调白细胞减少症模型小鼠血清中亚精胺、尿酸、柠檬酸、烟酰胺、二十碳五烯酸、亚油酸、芥酰胺、1-磷酸鞘氨醇等8个差异代谢物的含量,其作用涉及亚油酸代谢途径、烟酸和烟酰胺代谢途径、三羧酸循环途径。结论:黄芪注射液中次生成分具有升高白细胞减少症模型小鼠白细胞的作用,其作用机制可能与干预亚油酸代谢途径、烟酸和烟酰胺代谢途径、三羧酸循环途径有关。
ABSTRACT: OBJECTIVE:To study metabonomics of secondary components of Astragalus membranaceus injection on leukopenia model mice. METHODS :The mice were divided into normal group ,model group ,A. membranaceus injection group (0.004 mL/g),secondary components low-dose ,medium-dose and high-dose groups (0.004,0.008,0.016 mL/g),with 7 mice in each group. Except for normal group ,other groups were given cyclophosphamide (80 mg/kg) intraperitoneally to induce leukopenia model. After modeling ,administration groups were given relevant medicine intraperitoneally ,and normal group and model group were given constant volume of sterile water for injection intraperitoneally ,once a day ,for consecutive 7 days. During the experiment ,the changes of body weight of mice were measured every 2 days. After the last administration ,the blood routine indexes [white blood cell (WBC),neutrophil(NE),lymphocyte(LY)and monocyte (MO)counts] of mice were detected by animal blood analyzer. UPLC-Q Exactive Orbitrap-HRMS combined with multivariate statistical analysis were used to analyze the metabonomics of mice serum. RESULTS :Compared with model group ,body weight of mice in the secondary component low-dose group increased significantly on the 4th and 8th day of administration (P<0.05),and the counts of WBC ,NE,LY and MO in serum of mice in secondary component groups increased significantly (P<0.05 or P<0.01). Metabonomic analysis showed that the secondary components of A. membranaceus injection could significantly regulate the contents of 8 endogenous metabolites in serum, including spermidine , uric acid , citric acid , nicotinamide, eicosapentaenoic acid , linoleic acid , erucamide and sphingosine-1-phosphate. Their effects involved linoleic acid metabolism pathway ,nicotinic acid and nicotinamide metabolism pathway and tricarboxylic acid cycle pathway. CONCLUSIONS :The secondary components of A. membranaceus injection possess the effect of increasing white blood cells in leukopenia model mice ,the mechanism of which may be related to intervention of linoleic acid metabolism pathway , nicotinic acid and nicotinamide metabolism pathway ,and tricarboxylic acid cycle pathway.
期刊: 2021年第32卷第17期
作者: 曲婷丽,刘腾,郑茜,卢晓林,赵正保,李震宇
AUTHORS: QU Tingli,LIU Teng,ZHENG Qian,LU Xiaolin ,ZHAO Zhengbao ,LI Zhenyu
关键字: 黄芪注射液;次生成分;代谢组学;白细胞减少症
KEYWORDS: Astragalus membranaceus injection;
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