车前子多糖对乳腺癌细胞增殖、迁移和侵袭的影响及其机制
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篇名: 车前子多糖对乳腺癌细胞增殖、迁移和侵袭的影响及其机制
TITLE: Effect and Its Mechanism of Plantago asiatica Polysaccharide on the Proliferation ,Migration and Invasion of Breast Cancer Cells
摘要: 目的:研究车前子多糖对乳腺癌细胞增殖、迁移和侵袭的影响,并初步探究其作用机制。方法:以人乳腺癌细胞MDA-MB-231为对象,采用MTT法检测不同质量浓度车前子多糖(8、16、32、64mg/L)对细胞增殖能力的影响并计算细胞存活率;采用划痕实验、Transwell侵袭实验分别检测不同质量浓度车前子多糖(8、16mg/L)对细胞迁移和侵袭能力的影响;采用Westernblot法检测转移及上皮间质转化(EMT)相关蛋白[基质金属蛋白酶2(MMP-2)、MMP-9、上皮黏钙素(E-cadherin)、神经黏钙素(N-cadherin)、波形蛋白(vimentin)]的表达情况。结果:MTT实验结果显示,车前子多糖32、64mg/L组细胞的存活率均较对照组显著降低(P<0.05或P<0.01),故以不影响细胞存活率的8、16mg/L作为后续实验给药浓度。与对照组比较,车前子多糖8、16mg/L组细胞的相对迁移率(12、24h)、相对侵袭率以及MMP-2、MMP-9、N-cadherin、vimentin蛋白的相对表达量均显著降低(P<0.05或P<0.01),E-cadherin蛋白的相对表达量均显著升高(P<0.05或P<0.01)。结论:车前子多糖能够在体外抑制乳腺癌细胞MDA-MB-231的增殖,并可能通过抑制转移及EMT相关蛋白的表达来抑制该细胞的迁移和侵袭。
ABSTRACT: OBJECTIVE:To study the effects of Plantago asiatica polysaccharide on the proliferation ,migration and invasion of breast cancer cells ,and to investigate its mechanism preliminarily. METHODS :Using human breast cancer cell MDA-MB- 231 as subjects ,MTT method was adopted to detect the effects of different concentrations of P. asiatica polysaccharide(8,16,32,64 mg/L)on the cell proliferation ability ,and survival rate of the cells was calculated. Scratch test and Transwell invasion test were used to detect the effects of different concentrations of P. asiatica polysaccharide(8,16 mg/L)on cell migration ability and invasion ability. Western blot assay was used to detect the expression of epithelial-mesenchymal transition (EMT)-related proteins [matrix metalloproteinase- 2(MMP-2),MMP-9,E-cadherin,N-cadherin,vimentin]. RESULTS :Results of MTT assay showed that survival rate of the cells in 32,64 mg/L P. asiatica polysaccharide groups were significantly lower than control group (P<0.05 or P<0.01),so that 8,16 mg/L,which did not affect the cell survival rate ,were used as the follow-up drug concentrations. Compared with control group ,relative mobility (12,24 h),relative invasion rate and relative expression of MMP- 2,MMP-9, N-cadherin and vimentin protein were decreased significantly in 8,16 mg/L P. asiatica polysaccharide groups (P<0.05 or P< 0.01),while relative expression of E-cadherin protein was increased significantly (P<0.05 or P<0.01). CONCLUSIONS :P. asiatica polysaccharide can inhibit the proliferation of breast cancer cells MDA-MB- 231,and inhibit the migration and invasion of the cells by regulating the expression of metastasis and EMT-related proteins.
期刊: 2021年第32卷第15期
作者: 陈高,杨晓婷,王曦,金湛,朱瑞,甘椿椿,周道堂,姚水洪
AUTHORS: CHEN Gao,YANG Xiaoting ,WANG Xi,JIN Zhan,ZHU Rui,GAN Chunchun ,ZHOU Daotang ,YAO Shuihong
关键字: 车前子多糖;乳腺癌;MDA-MB-231细胞;增殖;迁移;侵袭
KEYWORDS: Plantago asiatica polysaccharide;Breast cancer ;MDA-MB-231 cells;Proliferation;Migration;Invasion
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