壮药三七姜乙酸乙酯提取物抗炎、镇痛作用的谱-效关系研究
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篇名: 壮药三七姜乙酸乙酯提取物抗炎、镇痛作用的谱-效关系研究
TITLE: Study on Spectrum-effect Relationship of Anti-inflammatory and Analgesic Effects of Ethyl Acetate Extract from Zhuang Medicine Stahlianthus involucratus
摘要: 目的:考察不同产地壮药三七姜乙酸乙酯提取物抗炎、镇痛作用的谱-效关系。方法:以不同产地的10批三七姜为样品,分别以二甲苯致小鼠耳廓肿胀、乙酸致小鼠扭体反应实验考察其乙酸乙酯提取物的抗炎、镇痛活性。采用《中药色谱指纹图谱相似度评价系统(2012版)》建立10批三七姜乙酸乙酯提取物的高效液相色谱指纹图谱并进行相似度评价,通过与对照品比对进行共有峰的指认。基于Pearson相关系数法(以小鼠耳肿胀度和15min内扭体次数为药效指标)和灰色关联分析法(以耳肿胀抑制率和镇痛率为药效指标)分析三七姜乙酸乙酯提取物抗炎、镇痛的谱-效关系。结果:10批不同产地三七姜乙酸乙酯提取物均具有较好的抗炎、镇痛作用,其对小鼠的耳肿胀抑制率为46.43%~55.16%,对小鼠的镇痛率为45.56%~52.72%。10批样品共确定了18个共有峰,其与对照指纹图谱的相似度为0.994~0.997。通过与对照品比对,指认出了峰1、2、4分别为原儿茶酸、对羟基苯甲酸、对羟基苯甲醛。Pearson相关性分析结果显示,峰10、18与小鼠耳肿胀度和15min内扭体次数均呈显著负相关(r分别为-0.853、-0.738,P分别为0.002、0.015);灰色关联分析结果显示,18个共有峰与耳肿胀抑制率和镇痛率的关联度均在0.65以上,其中峰14、1(原儿茶酸)、17、9、4(对羟基苯甲醛)、2(对羟基苯甲酸)、16、7、6与耳肿胀抑制率的关联度相对较高(关联度均高于0.7),峰1(原儿茶酸)、17、14、9、16、2(对羟基苯甲酸)、4(对羟基苯甲醛)与镇痛率的关联度相对较高(关联度均高于0.7)。结论:三七姜乙酸乙酯提取物具有较好的抗炎、镇痛作用,峰1(原儿茶酸)、2(对羟基苯甲酸)、10、14、17、18等可能是其主要活性成分。
ABSTRACT: OBJECTIVE:To i nvestigate the spectrum-effect relationship of analgesic and anti-inflammatory effects of ethyl acetate extract from Zhuang medicine Stahlianthus involucratus from different habitats. METHODS :Ten batches of S. involucratus from different habitats were used as samples to investigate the anti-inflammatory and analgesic activities of ethyl acetate extracts by xylene induced ear swelling test and acetic acid induced writhing test in mice. HPLC fingerprints of 10 batches of ethyl acetate extract from S. involucratus were established and their similarity was evaluated by using Similarity Evaluation System of TCM Chromatogram Fingerprint (2012 edition),and the common peaks were identified by comparison with the control. The spectrum-effect relationship of anti-inflammatory and analgesic effects of ethyl acetate extract from S. involucratus were analyzed on the basis of Pearson correlation coefficient (auricle swelling degree and writhing times in 15 min as pharmacodynamic indexes )and Grey relational analysis (inhibition rate of ear swelling and analgesic rate as pharmacodynamic indexes ). RESULTS : batches of ethyl acetate extract from S. involucratus had obvious anti-inflammatory and analgesic effects ; inhibition rates of ear swelling in mice were 46.43%-55.16%,and the analgesic rates of mice were 45.56%-52.72%. A total of 18 common peaks were identified in 10 batches of samples ,andthe similarity between them and the control fingerprint was 0.994-0.997. Compared with substance control ,the pea ks 1,2 and 4 were identified as protocatechuic acid , p-hydroxy- 0771-4953513。E-mail:liangjie1101@126.com benzoic acid and p-hydroxybenzaldehyde,respectively. Results of Pearson correlation analysis showed that peak 10 and peak 18 were significantly negative correlated with auricle swelling degree and writhing times in 15 min(r were values -0.853,-0.738,P values were 0.002,0.015,respectively). Results of Gray correlation degree analysis showed that the correlation degree of 18 common peaks with inhibition rate of ear swelling and analgesic rate were all greater than 0.65;among them ,peaks 14,1(protocatechuic acid ),17,9,4(p-hydroxybenzaldehyde),2(p-hydroxybenzoic acid ), 16,7 and 6 showed the relatively high correlation degree (correlation degree >0.7);peak 1(protocatechuic acid ),17,14,9,16,2 (p-hydroxybenzoic acid )and 4(p-hydroxybenzaldehyde)showed the relatively high correlation degree (correlation degree >0.7). CONCLUSIONS:The ethyl acetate extract of S. involucratus show good anti-inflammatory and analgesic effects. Peak 1 (protocatechuic acid ),2(p-hydroxybenzoic acid ),10,14,17,18 may be its main active ingredients.
期刊: 2021年第32卷第14期
作者: 梁洁,陈辉华,杨川川,曹玉嫔,陈俊,李耀华,陈晓思,柳贤福
AUTHORS: LIANG Jie, CHEN Huihua,YANG Chuanchuan,CAO Yupin,CHEN Jun,LI Yaohua,CHEN Xiaosi,LIU Xianfu
关键字: 三七姜;乙酸乙酯提取物;抗炎;镇痛;谱-效关系;Pearson相关性分析;灰色关联分析
KEYWORDS: Stahlianthus involucratus ;Ethyl acetate extract ;Anti-inflammatory;Analgesic;Spectrum-effect relationship ;
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