草果酚酸的提取工艺优化
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篇名: 草果酚酸的提取工艺优化
TITLE: Optimization of the Extraction Technology of Phenolic Acid from Amomum tsaoko
摘要: 目的:优化草果酚酸的提取工艺。方法:以乙醇体积分数、液料比、提取时间为考察因素,原儿茶酚酸和香草酸的总含量为响应值,采用Box-Behnken设计-响应曲面法优化草果酚酸的提取工艺并验证。结果:最优提取工艺为乙醇体积分数65%,液料比4∶1(mL/g),提取时间2.5h。经3次实验验证,原儿茶酚酸和香草酸的平均总含量为12.32mg/g(RSD=0.26%,n=3),与预测值(12.63mg/g)的平均相对误差为2.45%。结论:优化所得工艺稳定、可行。
ABSTRACT: OBJECTIVE:To opt imize the extraction technology of phenolic acid from Amomum tsaoko . METHODS :The extraction technology of phenolic acid from A. tsaoko was optimized by using Box-Behnken design-response surface methodology with ethanol volume fraction ,liquid-solid ratio and extraction time as factors ,using the total contents of protocatechuic acid and vanillic acid as response value. The optimizd extraction technology was vlidated. RESULTS :The optimal extraction technology was as follows :ethanol volume fraction 65%,liquid-solid ratio 4∶1(mL/g),extraction time 2.5 h. After 3 times of validation tests , average total content of protocatechuic acid and vanillic acid were 12.32 mg/g(RSD=0.26 %,n=3),average relative error of which with predicted value (12.63 mg/g)was 2.45%. CONCLUSIONS :The optimal technology is stable and feasible .
期刊: 2021年第32卷第14期
作者: 沈华,管红梅,钟燕,唐艺玲,胥鑫,代敏,蒲忠慧
AUTHORS: SHEN Hua,GUAN Hongmei ,ZHONG Yan,TANG Yiling,XU Xin,DAI Min,PU Zhonghui
关键字: 草果酚酸;提取工艺优化;Box-Behnken设计-响应曲面法;原儿茶酚酸;香草酸
KEYWORDS: Phenolic acid from Amomum tsaoko ;Extraction technology optimization ;Box-Behnken design-response surface
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