β-乳香酸对海马神经元细胞氧糖剥夺损伤的改善作用
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篇名: β-乳香酸对海马神经元细胞氧糖剥夺损伤的改善作用
TITLE: Improvement Effects of β-boswellic Acid on Hippocampal Neurons Cells Injury Induced by Oxygen-glucose Deprivation
摘要: 目的:研究β-乳香酸对大鼠海马神经元细胞氧糖剥夺损伤的改善作用。方法:将大鼠海马神经元细胞分为正常对照组、模型组和β-乳香酸低、中、高浓度组(1、10、100μmol/L),除正常对照组外,其余各组细胞加入相应含药培养基,并进行氧糖剥夺培养以复制氧糖剥夺损伤模型。采用MTT法检测细胞的存活率,采用化学比色法检测细胞上清液中乳糖脱氢酶(LDH)活性,采用Hoechst-PI染色法观察细胞形态的变化,采用流式细胞仪检测细胞早期凋亡率,采用Westernblot法检测细胞中凋亡相关蛋白[B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)、激活型胱天蛋白酶3(cleavedcaspase-3)]的表达。结果:与模型组比较,β-乳香酸中、高浓度组细胞存活率、Bcl-2蛋白表达水平均显著升高(P<0.01),细胞上清液中LDH活性、细胞早期凋亡率和细胞中cleavedcas-pase-3、Bax蛋白表达水平均显著降低(P<0.05或P<0.01);细胞核致密浓染、碎片状现象明显减少。结论:β-乳香酸可改善大鼠海马神经元细胞氧糖剥夺损伤,其作用机制可能与下调cleavedcaspase-3、Bax蛋白表达,上调Bcl-2蛋白表达有关。
ABSTRACT: OBJECTIVE:To study the imp rovement effects of β-boswellic acid on hippocampal neurons cells injury of rats induced by oxygen-glucose deprivation. METHODS :The hippocampal neurons cell of rats were divided into normal control group , model group and β-boswellic acid low-concentration ,medium-concentration and high-concentration groups (1,10,100 μmol/L). Except for normal control group ,other groups were cultured with relevant medium and given oxygen glucose deprivation to induce oxygen-glucose deprivation induced injury model. MTT assay was adopted to detect cell viability. Chemical colorimetry was used to detect LDH activity in cell culture supernatant. Hoechst-PI staining was used to detect the morphology change of cells. Flow cytometry was used to detect early apoptosis rate of cells. The expression of apoptosis-related protein (Bcl-2,Bax and cleaved caspase-3) were detected by Western blot. RESULTS :Compared with model group ,the survival rate of cells and protein expression of Bcl- 2 were increased significantly in β-boswellic acid medium-concentration and high-concentration groups (P< 0.01),while LDH activity ,early apoptosis rate ,protein expression of cleaved caspase- 3 and Bax were all decreased significantly (P<0.05 or P<0.01). The densely stained nuclei and fragmentation decreased significantly. CONCLUSIONS :β-boswellic acid can relieve oxygen-glucose deprivation induced injury of hippocampal neurons cells ,the mechanism of which may be associated with down-regulating the protein expression of cleaved caspase- 3 and Bax and up-regulating the protein expression of Bcl- 2.
期刊: 2021年第32卷第11期
作者: 王明明,王磊,窦芳,李韦韦,文爱东,王婧雯
AUTHORS: WANG Mingming,WANG Lei,DOU Fang,LI Weiwei,WEN Aidong,WANG Jingwen
关键字: β-乳香酸;海马神经元细胞;氧糖剥夺;细胞凋亡
KEYWORDS: β-boswellic acid ;Hippocampal neurons cells ;Oxygen-glucose deprivation ;Cell apoptosis
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