不同干燥方式对连翘花主要成分的影响
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篇名: 不同干燥方式对连翘花主要成分的影响
TITLE: Effects of Different Drying Methods on the Main Components of Forsythia suspensa Flowers
摘要: 目的:建立测定阴干、真空冷冻干燥、烘箱干燥(30、50、70℃)、晒干等干燥方式下连翘花中4种成分含量的方法并进行比较,以评价不同干燥方式对连翘花主要成分含量的影响,筛选其最佳干燥方式。方法:采用超高效液相色谱法。色谱柱为WatersACQUITYUPLCBEHC18,流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),流速为0.3mL/min,检测波长为230nm,柱温为35℃,进样量为1μL。采用优劣解距离法(TOPSIS)综合分析法计算不同干燥方式与理想方法的欧氏贴近度(C),i以确定最佳干燥方法。结果:连翘酯苷A、芦丁、连翘酯苷、(+)-松脂素-4-O-β-D-葡萄吡喃糖苷检测进样量的线性范围分别为0.0075~0.0377、0.0274~0.1372、0.0019~0.0095、0.0056~0.0288µg(r均大于0.999);精密度、稳定性(32h)、重复性试验的RSD均小于2%;加样回收率分别为97.27%~102.53%、100.53%~104.11%、98.45%~104.02%、98.66%~104.82%,RSD均小于3%(n=3)。含量分别为1.6458~4.9879、11.7302~20.9780、0.8755~2.0050、2.3660~5.5357mg/g。连翘酯苷A以30℃烘箱干燥后含量最高,芦丁、(+)-松脂素-4-O-β-D-葡萄吡喃糖苷均以真空冷冻干燥后含量最高,连翘苷以50℃烘箱干燥后含量最高。TOPSIS分析结果显示,阴干、真空冷冻干燥、烘箱干燥(30、50、70℃)、晒干的Ci分别为0.0799、0.5535、0.4954、0.5038、0.1579、0.2172;Ci排序为真空冷冻干燥>50℃烘箱干燥>30℃烘箱干燥>晒干>70℃烘箱干燥>阴干。结论:所建含量测定方法简便、重复性好,可用于同时测定连翘花中4种成分的含量。连翘花样品以真空冷冻干燥方式最佳,其次为50℃烘箱干燥、30℃烘箱干燥,再次为晒干和烘箱干燥70℃,最后为阴干。
ABSTRACT: OBJECTIVE:To establish the method for the content det ermination of 4 components in Forsythia suspensa flowers by drying in shade ,vacuum freeze-drying ,oven(30,50,70 ℃)and sun ,so as to evaluate the effects of different drying methods on the main components of F. suspensa flowers and screen the optimal drying method. METHODS :UPLC method was adopted. The determination was performed on Waters ACQUITY UPLC BEH C 18 column with mobile phase consisted of acetonitrile- 0.1% phosphoric acid aqueous solution (gradient elution )at the flow rate of 0.3 mL/min. The detection wavelength was set at 230 nm, and column was 35 ℃. The sample size was 1 μL. Euclidean closeness(C)i of different drying methods was calculated by TOPSIS comprehensive analysis method ,and the optimal drying method was defined. RESULTS :The linear range of forsythiaside A , rutin,forsythin,(+)-pinoresinol-4-O-β-D-glucopyranoside were 0.007 5-0.037 7,0.027 4-0.137 2,0.001 9-0.009 5,0.005 6-0.028 8 µg(all r>0.999). RSDs of precision ,stability(32 h)and reproducibility tests were all lower than 2%. The recoveries were 97.27%-102.53%,100.53%-104.11%,98.45%-104.02%,98.66%-104.82%,respectively;and all RSDs <3%(n=3). The contents were 1.645 8-4.987 9,11.730 2-20.978 0,0.875 5-2.005 0,2.366 0-5.535 7 mg/g. The content of forsythiaside A was the highest after drying at 30 ℃,rutin and (+)-pinoresinol-4- O-β-D-glucopyranoside were the highest after vacuum freeze-drying,forsythiaside was the highest after drying at 50 ℃ . Results of TOPSIS analysis showed that Ci of F. suspensa flowers by drying in shade ,vacuum freeze-drying ,oven(30,50,70 ℃)and sun were 0.079 9,0.553 5,0.495 4, 0.503 8,0.157 9,0.217 2,respectively;the order of Ci was vacuum freeze-drying > 50 ℃ oven drying > 30 ℃ oven drying>sun drying >70 ℃ oven drying > shade drying. CONCLUSIONS:Established method is simple ,reproducible and can be used for the content determination of 4 components in F. suspensa flowers. The samples are preferably dried by vacuum freeze-drying,followed by 50 ℃ oven drying ,30 ℃ oven drying , and then dried in the sun and oven at 70 ℃ and finally in the shade.
期刊: 2021年第32卷第08期
作者: 杨梦婷,王相,王倩,王鑫国,毕晓东,牛丽颖
AUTHORS: YANG Mengting ,WANG Xiang,WANG Qian,WANG Xinguo ,BI Xiaodong ,NIU Liying
关键字: 连翘花;主要成分;阴干;烘箱干燥;真空冷冻干燥;晒干
KEYWORDS: Forsythia suspensa flowers;Main component;Drying in the shade ;Oven drying ;Vacuum freeze-drying ;Drying
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