“汗渍法”炮制对了哥王中芫花素含量及其抗氧化能力的影响
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篇名: “汗渍法”炮制对了哥王中芫花素含量及其抗氧化能力的影响
TITLE: Effects of “Sweat Soaking Method ”on the Content of Genkwanin in Wikstroemia indica and Its Anti-oxidation Ability
摘要: 目的:比较“汗渍法”炮制前后了哥王乙醇提取物中活性/毒性成分芫花素的含量变化,以及炮制对其抗氧化能力的影响。方法:采用高效液相色谱法测定“汗渍法”炮制前后了哥王药材中芫花素的含量。色谱柱为DiamonsilC18,流动相为0.2%磷酸水溶液-甲醇(梯度洗脱),流速为1mL/min,柱温为30℃,检测波长为346nm,进样量为20µL。将SD大鼠随机分为空白组、了哥王生品乙醇提取物组(317.52mg/kg,简称“生品组”)和了哥王炮制品乙醇提取物组(317.52mg/kg,简称“炮制品组”),每组6只。空白组大鼠灌胃等体积1.0%羧甲基纤维素钠溶液,各给药组灌胃相应药物混悬液,灌胃体积均为20mL/kg,每日1次,连续14d。采用酶联免疫吸附测定法检测各组大鼠血清氧化应激指标[丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)]的含量。结果:芫花素进样检测量的线性范围为0.147~27.360μg(r=0.9999);精密度、重复性、稳定性试验的RSD均小于3%;平均加样回收率为98.64%~98.92%(RSD<1%,n=3)。“汗渍法”炮制前后,了哥王药材中芫花素的平均含量分别为0.3776、0.2340mg/g。与空白组比较,生品组大鼠血清中SOD含量显著升高,CAT含量显著降低(P<0.05或P<0.01);炮制品组大鼠血清中MDA含量显著降低,SOD含量显著升高(P<0.05或P<0.01);且炮制品组MDA含量显著低于生品组,SOD含量显著高于生品组(P<0.05)。结论:经“汗渍法”炮制后,了哥王药材中芫花素的含量有所降低,且抗氧化活性增强;“汗渍法”具有一定的“减毒增效”效果。
ABSTRACT: OBJECTIVE:To compare the content changes of active/toxic ingredient genkwanin in ethanol extract from Wikstroemia indica before and after processing with “sweat soaking method ”and the effects of processing method on its anti-oxidation ability. METHODS :HPLC method was adopted to determine the content of genkwanin in W. indica before and after processing with “sweat soaking method ”. The separation was performed on Diamonsil C 18 column with 0.2% phosphoric acid solution-methanol as mobile phase (gradient elution )at the flow rate of 1 mL/min. The column temperature was 30 ℃ and detection wavelength was set at 346 nm. The sample size was 20 µL. SD rats were randomly divided into blank group ,W. indica raw product ethanol extract group (317.52 mg/kg,called“raw-product group ”as short )and W. indica processed product ethanol extract group (317.52 mg/kg,called“processed-product group ”as short ),with 6 rats in each group. Blank group was given constant volume of 1.0%CMC-Na solution intragastrically ,and administration groups were given relevant medicine suspension intragastrically;all of them were given 20 mL/kg,once a day ,for consecutive 14 days. The contents of serum oxidant stress indexes(MDA,CAT,SOD)in rats were determined by ELISA. RESULTS :The linear range of genkwanin were 0.147-27.360 μg (r=0.999 9);RSDs of precision ,reproducibility and stability tests were all lower than 3% ;average recoveries were 98.64%-98.92%(RSD<1%,n=3). Before and after processing with “sweat soaking method ”,average contents of genkwanin in W. indica were 0.377 6 and 0.234 0 mg/g. Compared with blank group ,the serum content of SOD in raw-product group was increased significantly ,while CAT content was decreased significantly (P<0.05 or P<0.01);the serum content of MDA was decreased significantly in processed-product group ,while SOD content was increased significantly (P<0.05 or P<0.01). MDA content of processed-product group was significantly lower than that of raw-product group ,while SOD content was significantly higher than raw-product group (P<0.05). CONCLUSIONS :After proce ssing with “sweat soaking method ”,the content of genkwanin in W. indica is decreased ,and antioxidant activity is increased .“Sweat soaking method ”processes certain function of “reducing toxicity and increasing efficiency ”.
期刊: 2020年第31卷第19期
作者: 周志容,冯果,李玮,郑传奇,许琴,任晨晨,彭礼珍,黄侨宗,肖晓燕
AUTHORS: ZHOU Zhirong,FENG Guo,LI Wei,ZHENG Chuan qi,XU Qin,REN Chenchen,PENG Lizhen,HUANG Qiaozong,XIAO Xiaoyan
关键字: 了哥王;汗渍法;芫花素;含量;抗氧化能力;减毒增效
KEYWORDS: Wikstroemia indica ;Sweat soaking method ;Genkwanin;Content;Anti-oxidation ability ;Reducing toxicity and
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