不同产地茯苓中7种三萜类成分含量的测定及聚类分析
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篇名: 不同产地茯苓中7种三萜类成分含量的测定及聚类分析
TITLE: Content Determination and Cluster Analysis of 7 Kinds of Triterpenes in Poria cocos from Different Habitats
摘要: 目的:建立同时测定茯苓药材中7种三萜类成分含量的方法,并比较不同产地茯苓药材中上述成分的差异性,为茯苓药材的质量控制提供参考。方法:以不同产地的36批茯苓药材为样品,采用高效液相色谱法测定去氢土莫酸、猪苓酸C、3-表去氢土莫酸、3-O-乙酰基-16α-羟基-氢化松苓酸、去氢茯苓酸、茯苓酸、松苓新酸的含量。色谱柱为ThermoAcclaim120C18,流动相为乙腈-磷酸水(梯度洗脱),流速为1mL/min,检测波长为210nm,柱温为30℃,进样量为20μL。采用SPSS21.0统计学软件对36批不同产地茯苓药材进行聚类分析。结果:7种三萜类成分在各自质量浓度范围内线性关系均良好(r均不低于0.9990),平均加样回收率为96.74%~104.04%(RSD为0.54%~1.55%,n=6);精密度、重复性、稳定性(24h)试验的RSD均小于3.0%(n=6);耐用性试验的RSD均小于5.0%(n=2)。不同产地样品之间7个指标成分的单一含量均存在一定差异,但总体差异不明显(多数样品总含量分布在1.3~1.9mg/g之间)。经聚类分析,36批样品共聚为5类,即S27单独聚为第Ⅰ类,S30、S34聚为第Ⅱ类,S2、S8、S9聚为第Ⅲ类,S10、S11、S12、S14聚为第Ⅳ类,其余26批样品聚为第Ⅴ类。结论:该方法操作简单,精密度、重复性、耐用性均良好,可用于同时测定茯苓药材中上述7种三萜类成分的含量。各产地茯苓药材的质量总体差异不显著,大部分产区的茯苓含量均一、质量稳定,仅少部分存在差异。
ABSTRACT: OBJECTIVE:To establish a method for th e content determination of 7 kinds of triterpenoids in Poria cocos ,and to compare the differences of the above components in P. cocos from different habitats ,so as to provide reference for the quality control of P. cocos . METHODS :Using 36 batches of P. cocos from different habitats as samples ,HPLC method was used for content determination of dehydrotomorphic acid , polyporhinic acid C , 3-epidehydrotomorphic acid , 3-O-acetyl-16 α-hydroxy-hydrogenolysaccharic acid ,dehydrotomorphic acid ,pachymic acid and dehydrotrametenolic acid. The column was performed on Thermo Acclaim 120 C18 with mobile phase consisted of acetonitrile-phosphoric acid water (gradient elution )at the flow rate of 1 mL/min. The detection wavelength was set at 210 nm. The column temperature was 30 ℃,and the injection volume was 20 μL. SPSS 21.0 statistical software was used for cluster analysis of 36 batches of P. cocos from different habitats. RESULTS : The linearity of 7 triterpenoids was good in the range of their mass concentration (all r≥0.999 0);average recoveries were 96.74%-104.04%(RSD=0.54%-1.55%,n=6). RSDs of precision ,and reproducibility stability (24 h)tests were all lower than 3.0%(n=6). RSD of durability test was lower than 5.0%(n=2). There were some differences in the single content of 7 indicator components among samples from different habitats ,but the total content difference was not obvious (the total content of most samples was in the range of 1.3-1.9 mg/g). After cluster analysis ,36 batches of sample were clustered into 5 categories,i.e. S 27 was clustered into class Ⅰ;S30 and S 34 were clustered into class Ⅱ;S2,S8 and S 9 were clustered into class Ⅲ;S10,S11,S12 and S 14 were clustered into class Ⅳ;and the remaining 26 batches of samples were clustered into class Ⅴ. CONCLUSIONS :The method is simple ,and has good precision ,repeatability and durability. It can be used for the simultaneous determination of above 7 components in P. cocos . There has no significant difference in the quality of P. cocos from different habitats. The content of P. cocos in most producing areas is uniform in content and stable in quality ,only a few of them are different. Δ 基金项目 :国家重点研发计划中医药现代化研究重点专项
期刊: 2020年第31卷第17期
作者: 万鸣,黄超,杨玉莹,罗心遥,叶晓川,丁影,董琼,陈树和
AUTHORS: WAN Ming,HUANG Chao,YANG Yuying,LUO Xinyao,YE Xiaochuan,DING Ying,DONG Qiong,CHEN Shuhe
关键字: 茯苓;三萜类成分;高效液相色谱法;含量测定;聚类分析
KEYWORDS: Poria cocos ;Triterpenoids;HPLC;Content
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