基于古法特色炮制前后的地黄饮片及其多糖对衰老模型大鼠的抗氧化作用比较
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篇名: 基于古法特色炮制前后的地黄饮片及其多糖对衰老模型大鼠的抗氧化作用比较
TITLE: Comparison of Antioxidant Effect of Rehmannia glutinosa Decoction Pieces and Its Polysaccharides on Aging Model Rats before and after Processing Based on the Characteristic Ancient Method
摘要: 目的:比较地黄特色炮制过程中鲜地黄、干地黄、熟地黄及其炮制前后多糖对衰老模型大鼠的抗氧化作用差异,为地黄的加工炮制提供参考。方法:按照古法特色炮制方法制备熟地黄样品,保留加工过程中鲜地黄、干地黄样品,并采用水提醇沉法提取鲜地黄和熟地黄中的粗多糖。将96只大鼠分为空白组(水)、模型组(水)、阳性对照组[维生素C,100mg/(kg·d)]、鲜地黄组[700mg/(kg·d)]、干地黄组[135mg/(kg·d)]、熟地黄组[135mg/(kg·d)]、鲜地黄多糖组[1400mg/(kg·d),以鲜地黄质量计]和熟地黄多糖组[270mg/(kg·d),以熟地黄质量计],每组12只。除空白组外,其余各组大鼠均于颈背部皮下注射D-半乳糖[125mg/(kg·d)]复制亚急性衰老模型,并于造模同时灌胃给药,每日给药1次,连续给药56d。末次给药后,测定大鼠肝、脑、肾、脾、心脏、胸腺指数,并测定其血清和肝、脑、肾组织中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力、丙二醛(MDA)含量。结果:与空白组比较,模型组大鼠各脏器指数以及血清和脑、肝、肾组织中T-AOC和SOD活力、CAT活力均显著降低(P<0.05或P<0.01),MDA含量均显著升高(P<0.01)。与模型组比较,鲜地黄组大鼠脑、肝、肾指数以及血清和肾组织中SOD活力升高不显著(P>0.05);干地黄组大鼠肾指数以及血清和脑组织中T-AOC,血清和肝、肾组织中SOD活力升高不显著(P>0.05);鲜地黄多糖组大鼠肾指数以及血清和脑组织中T-AOC,血清中SOD活力升高不显著(P>0.05);其余各组大鼠脏器指数,血清和组织中T-AOC、SOD、CAT活力均显著升高(P<0.05或P<0.01);各给药组大鼠血清及脑、肝、肾组织中MDA含量均显著降低(P<0.05或P<0.01)。与鲜地黄组比较,干地黄组大鼠血清中T-AOC显著降低(P<0.01),其余测定指标差异无统计学意义(P>0.05);熟地黄组大鼠肾、脾指数显著升高(P<0.05),肾组织中T-AOC,血清和脑、肾组织中SOD活力,脑、肝组织中CAT活力均显著升高(P<0.05或P<0.01),脑、肝组织中MDA含量显著降低(P<0.01);熟地黄组大鼠脑和肝组织中CAT活力显著高于阳性对照组(P<0.01)。与鲜地黄多糖组比较,熟地黄多糖组大鼠脾、肾指数显著升高(P<0.05或P<0.01),血清和脑、肝、肾组织中T-AOC、SOD活力、CAT活力均显著升高(P<0.05或P<0.01)并且其脑、肝、肾组织中T-AOC和CAT活力均显著高于阳性对照组(P<0.05或P<0.01)。结论:在提高衰老模型大鼠脏器指数以及增强其血清和脑、肝、肾组织中抗氧化酶活力方面,熟地黄优于鲜地黄和干地黄,熟地黄多糖优于鲜地黄多糖。古法特色炮制地黄过程中多糖的改变提高了其抗氧化能力。
ABSTRACT: OBJECTIVE:To compare the difference in the antioxid ant effect of fresh Rehmannia glutinosa ,dried R. glutinosa , R. glutinosa preparata during ancient characteristic processing and its polysaccharides before and after processing on aging model rats,and to provide reference for the processing of R. glutinosa . METHODS :The sample of R. glutinosa preparata was prepared according to ancient characteristic method. During the processing ,the fresh and dried R. glutinosa samples were retained. Then crude polysaccharide were extracted from fresh R. glutinosa and Rehmanniae radix preparata by water extraction and alcohol precipitation. Totally 96 rats were divided into blank group (water),model group (water),positive control group [vitamine C ,100 mg/(kg·d)],fresh R. glutinosa group [ 700 mg/(kg·d)],dried R. glutinosa group [ 135 mg/(kg·d)] ,Rehmanniae radix preparata group [ 135 mg/(kg·d)],fresh R. glutinosa polysaccharide group [ 1 400 mg/(kg·d),by the weight of fresh R. glutinosa ] and Rehmanniae radix preparata polysaccharide group mg/(kg·d),by the weight of Rehmanniae radix preparata] , with 12 rats in each group. Except for blank group ,other 制。E-mail:zhouyan1221@163.com groups were given D-galactose [ 125 mg/(kg·d)] on neck and back to induce sub-acute aging model. At the same time ,they were given relevant medicine in tragastrically,once a day ,for consecutive 56 days. After last admin istration,the liver ,brain,kidney,spleen,heart and thymus indexes were determined. The total antioxidant capacity (T-AOC),superoxide dismutase (SOD)activity,catalase(CAT)activity and MDA content in serum , liver,brain and kidney were determined. RESULTS :Compared with blank group ,organ indexes of rats in the model group were decreased significantly (P<0.05 or P<0.01);T-AOC,SOD activity and CAT activity in serum ,brain,liver and kidney tissue were decreased significantly (P<0.01),while MDA content increased significantly (P<0.01). Compared with model group ,the organ indexes of brain ,liver and kidney ,SOD activity in serum and kidney of fresh R. glutinosa group were not significantly increased (P>0.05);kidney index ,T-AOC in serum and brain ,SOD activity in serum ,liver and kidney tissue were not significantly increased in the dried R. glutinosa group(P>0.05);kidney index ,T-AOC in serum and cerebral tissue ,SOD activity in serum were not significantly increased in fresh R. glutinosa group(P>0.05);other organ indexes ,T-AOC,SOD activity and CAT activity in serum and tissues were increased significantly in other groups (P<0.05 or P<0.01),while MDA content in serum and tissues were decreased significantly in all administration groups (P<0.05 or P<0.01). Compared with fresh R. glutinosa group,T-AOC in serum was decreased significantly in dried R. glutinosa group(P<0.01),and there was no significant difference in other indexes (P>0.05);kidney and spleen indexes of rats in Rehmanniae radix preparata group were increased significantly (P<0.05),T-AOC in renal tissue ,SOD activity in serum ,cerebral tissue and renal tissue ,CAT activity in cerebral and liver tissue were increased significantly (P<0.05 or P<0.01),while MDA in cerebral and liver tissue were significantly decreased (P< 0.01). CAT in cerebral tissue and liver tissue of rats in Rehmanniae radix preparata group were significantly higher than those in positive control group (P<0.01). Compared with fresh R. glutinosa polysaccharide group ,spleen and renal indexes of rats in Rehmanniae radix preparata group were increased significantly (P<0.05 or P<0.01),T-AOC,SOD activity and CAT activity in serum and cerebral ,liver,renal tissues were increased significantly (P<0.05 or P<0.01). T-AOC and CAT activity of cerebral , liver and renal tissues in Rehmanniae radix preparata group were all significantly higher than those in positive control group (P< 0.05 or P<0.01). CONCLUSIONS :In the aspect of increasing organ index and improving the activity of antioxidant enzymes in serum,cerebral,liver and
期刊: 2020年第31卷第17期
作者: 周艳,杜莉杰,郑旭亚,孙菲菲,张振凌
AUTHORS: ZHOU Yan,DU Lijie,ZHENG Xuya,SUN Feifei,ZHANG Zhenling
关键字: 熟地黄;古法特色炮制;地黄多糖;抗氧化能力;大鼠
KEYWORDS: Rehmanniae radix preparata ;Ancient characteristic processing ;Rehmannia glutinosa polysaccharides;Antioxidant
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