人参皂苷Rg1对局灶性脑缺血再灌注损伤模型大鼠的预防作用及机制研究
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篇名: 人参皂苷Rg1对局灶性脑缺血再灌注损伤模型大鼠的预防作用及机制研究
TITLE: Study on Preventive Effects and Mechanism of Ginsenoside Rg 1 on Focal Cerebral Ischemia-reperfusion Injury Model Rats
摘要: 目的:研究人参皂苷Rg1对局灶性脑缺血再灌注损伤(CIRI)模型大鼠的预防作用及机制。方法:将78只SD大鼠随机分为假手术组、模型组、丁苯酞对照组(阳性对照,10mL/kg)和人参皂苷Rg1低、中、高剂量组(10、20、40mg/kg),每组13只。各给药组大鼠腹腔注射相应药物,假手术组和模型组大鼠腹腔注射等体积生理盐水,每天1次,连续7d。给药结束后,除假手术组外,其余各组大鼠均采用右侧大脑中动脉栓塞法复制局灶性CIRI模型。造模完成后,参照改良神经功能缺损评分标准对大鼠进行神经功能缺损评分,采用2,3,5-氯化三苯基四氮唑(TTC)染色法测定大鼠脑梗死体积百分比,采用干湿重法测定大鼠脑含水量,采用酶联免疫吸附法检测大鼠血清中白细胞介素1β(IL-1β)、IL-6含量,分别采用免疫组织化学法和Westernblotting法测定大鼠脑组织中磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)、磷酸化核因子-κBp65(p-NF-κBp65)蛋白表达情况。结果:与假手术组比较,模型组大鼠神经功能缺失评分、脑梗死体积百分比、脑含水量和血清中IL-1β、IL-6含量以及脑组织中p-p38MAPK、p-NF-κBp65阳性蛋白表达细胞数和蛋白表达水平均显著升高(P<0.05或P<0.01);与模型组比较,各给药组大鼠上述指标水平均显著降低(P<0.05或P<0.01),且人参皂苷Rg1的作用具有一定的剂量依赖性趋势;人参皂苷Rg1中、高剂量组与丁苯酞对照组比较,上述指标差异均无统计学意义(P>0.05)。结论:人参皂苷Rg1对局灶性CIRI模型大鼠具有一定的预防作用。其机制可能与下调p-p38MAPK、p-NF-κBp65蛋白表达,抑制炎症因子IL-1β、IL-6的释放有关。
ABSTRACT: OBJECTIVE:To st udy preventive effect and mec hanism of ginsenoside Rg 1 on focal cerebral ischemia-reperfusion injury(CIRI)model rats. METHODS :Totally 78 SD rats were randomly divided into sham operation group ,model group , butylphthalide control group (positive control ,10 mL/kg),ginsenoside Rg 1 low-dose,medium-dose and high-dose groups (10, 20,40 mg/kg),with 13 rats in each group. Administration groups were give relevant medicine intraperitoneally ,sham operation group and model group were given constant volume of normal saline intraperitoneally ,once a day ,for consecutive 7 d. After medication,except for the sham operation group ,focal CIRI model was induced by middle cerebral artery occlusion (MCAO) method in other groups. After modeling ,neurological deficit scoring was performed according to the modified neurological difict scoring standard ; TTC staining was used to detected the percentage of cerebral infarction of rats ;the cerebral water content was measured by dry/wet weight method ;serum contents of IL- 1β and IL-6 were detected by ELISA ;the protein expressions of p-p 38 MAPK and p-NF-κB p65 in cerebral tissue were determined by immunohistochemistry and Western blotting assay. RESULTS : Compared with sham operation ,neurological deficits score ,percentage of cerebral infarction and cerebral water content ,serum contents of IL- 1β and IL-6,positive expression numbers of cells and protein expressions of p-p 38 MAPK and p-NF-κB p65 in cerebral tissue were increased significantly in model group (P<0.05 or P<0.01). Compared with model group ,above index levels of administration groups were all decreased significantly (P<0.05 or P<0.01),and the effect of ginsenoside Rg 1 had a dose-dependent trend ;there was no significant difference of all above indexes between ginsenoside Rg 1 middle-dose,high-dose groups and butylphthalide control group (P>0.05). CONCLUSIONS :Ginsenoside Rg 1 has a certain preventive effect on focal CIRI model rats ,the mechanism of which may be associated with down-regulating the protein expression of p-p 38 MAPK and p-NF-κB p65,inhibiting the release of inflammatory factors such as IL- 1β and IL-6.
期刊: 2020年第31卷第11期
作者: 武文文,吴诗卉,刘春红,包翠芬,闵连秋
AUTHORS: WU Wenwen ,WU Shihui ,LIU Chunhong ,BAO Cuifen ,MIN Lianqiu
关键字: 人参皂苷Rg1;磷酸化p38丝裂原活化蛋白激酶;磷酸化核因子-κB p65;脑缺血再灌注损伤;炎症;大鼠
KEYWORDS: Ginsenoside Rg 1;p-p38 MAPK;p-NF-κB p65;Cerebral ischemia-reperfusion injury ;Inflammation;Rats
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