Box-Behnken设计-响应面法优化小槐花中柠檬酚的超声辅助乙醇-硫酸铵双水相提取工艺
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篇名: Box-Behnken设计-响应面法优化小槐花中柠檬酚的超声辅助乙醇-硫酸铵双水相提取工艺
TITLE: Optimization of Ultrasonic-assisted Ethanol- (NH4)2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology
摘要: 目的:优化小槐花中柠檬酚的超声辅助乙醇-硫酸铵双水相提取工艺。方法:以柠檬酚含量为评价指标,乙醇体积分数、固液比、硫酸铵加入量、超声时间、超声温度为考察因素,在单因素试验的基础上,使用Box-Behnken设计-响应面法优化小槐花中柠檬酚的提取工艺并进行验证。结果:小槐花中柠檬酚的最优提取工艺为乙醇体积分数95.35%,固液比1∶50.35(g/mL),硫酸铵加入量4.49g,超声时间48.7min,超声温度57.6℃。3次验证试验中,柠檬酚的含量分别为0.6378、0.6384、0.6254mg/g,与预测值(0.6305mg/g)相近。结论:优化的超声辅助乙醇-硫酸铵双水相提取工艺稳定、可行,可用于小槐花中柠檬酚的提取。
ABSTRACT: OBJECTIVE:To optimize ultrasonic-assisted ethanol-(NH4)2SO4 aqueous two-phase extraction technology of citru- sinol from Desmodium caudatum . METHODS :Using the content of citrusinol as indexes ,with ethanol volume fraction , solid-liquid ratio ,(NH4)2SO4 addition amount ,ultrasonic time and ultrasonic temperature as factors ,based on the single factor tests,Box-Behnken design-response surface methodology was used to optimize the extraction technology of citrusinol. RESULTS : The optimized extraction technology of citrusinol included that ethanol volume fraction was 95.35%,the solid-liquid ratio was 1∶50.35 (g/mL),(NH4)2SO4 addition amount was 4.49 g,ultrasonic time was 48.7 min,ultrasonic temperature was 57.6 ℃. In 3 times of validation tests ,the extraction rates of citrusinol were 0.637 8,0.638 4,0.625 4 mg/g,respectively,which was close to predicted value(0.630 5 mg/g). CONCLUSIONS :The optimized ultrasonic-assisted ethanol- (NH4)2SO4 aqueous two-phase extraction technology is stable and feasible ,and can be used for the extraction of citrusinol from D. caudatum .
期刊: 2020年第31卷第09期
作者: 黄周锋,胡筱希,黄建猷,谭晓,陆国寿,梁新巧
AUTHORS: HUANG Zhoufeng ,HU Xiaoxi ,HUANG Jianyou ,TAN Xiao,LU Guoshou ,LIANG Xinqiao
关键字: 小槐花;柠檬酚;双水相提取;超声提取;Box-Behnken设计;响应面法;工艺优化
KEYWORDS: Desmodium caudatum ;Citrusinol;Aqueous two-phase extraction ;Ultrasonic extraction ;Box-Behnken design ;
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