药效学实验结合正交试验优选壮药柏金颗粒的水提醇沉工艺
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篇名: 药效学实验结合正交试验优选壮药柏金颗粒的水提醇沉工艺
TITLE: Optimization of the Water Extraction and Ethanol Pricipitation Technology of Zhuang Medicine Baijin Granules by Pharmacodynamics Combined with Orthogonal Test
摘要: 目的:优选壮药柏金颗粒的最佳水提醇沉工艺。方法:采用高效液相色谱法测定柏金颗粒组方药材中提取物中岩白菜素的含量。以浸膏得率结合小鼠降尿酸实验,初步筛选柏金颗粒的提取工艺路线(水煎煮、70%乙醇回流提取、水煎煮结合70%乙醇回流提取)。以浸膏得率和岩白菜素含量为指标,首先采用正交试验考察加水量、提取时间、提取次数等3个因素的影响,优化柏金颗粒的最佳水提工艺并进行验证试验;然后采用正交试验考察药液相对密度、含醇量、醇沉时间等3个因素的影响,优选柏金颗粒的最佳醇沉工艺并进行验证试验。通过小鼠降尿酸实验,比较醇沉处理前后柏金颗粒组方药材提取物的效果差异。结果:所建立的岩白菜素含量测定方法的线性范围为0.0072~0.288mg/mL,精密度、重复性、稳定性和准确度均良好。柏金颗粒的提取工艺初步筛选为水煎煮提取;最佳水提工艺为加入14倍量(mL/g)水,浸泡0.5h后,煎煮提取3次,每次1.0h;最佳醇沉工艺为水提液浓缩至相对密度1.0g/mL,药液含醇量60%,醇沉时间12h;验证试验均显示,所得浸膏得率及其岩白菜素含量的RSD均小于2%(n=3)。药效学实验结果显示,水提物(醇沉前)和水提醇沉物(醇沉后)均能显著降低高尿酸血症模型小鼠的尿酸水平(P<0.01),且两者降尿酸效果比较差异无统计学意义(P>0.05)。结论:采用优化的水提工艺可获得干浸膏得率和岩白菜素含量均较高的提取物,结合醇沉工艺除杂不影响其药效。该优化水提醇沉工艺可行,可用于柏金颗粒组方药材的提取、除杂。
ABSTRACT: OBJECTIVE:To optimize the water extraction and ethanol precipitation technology of Zhuang medicine Baijin granules. METHODS :HPLC method was adopted to determine the content of bergenin in Baijin granules extract. The extraction routes of Baijin granules (water decoction ,70% ethanol reflux extraction ,water decoction combined with 70% ethanol reflux extraction)was screened primarily with the yield of extract and the experiment of reducing uric acid of mice. The orthogonal test was adopted to optimize water extraction technology of Baijin granules with water multiple ,extraction time and extraction times as factors,taking the extraction yield and the bergenin content as index ,then the validation test was carried out. The orthogonal test was adopted to optimize the alcohol precipitation process of Baijin granules including the relative density of medicinal materials , alcohol content and the alcohol precipitation time ,then the validation test was carried out. By the experiment of reducing uric acid , the effects of medicinal materials extract of Baijin granules extract were compared before and after ethanol precipitation. RESLUTS:Established method for content determination of bergenin with linearity range of 0.007 2-0.288 mg/mL,had good precision,reproducibility,stability and accuracy. The initially chosen extraction process of Baijin granules was water decoction extraction. The optimal water extraction technology was soaked for 0.5 h,then decocted for 3 times with 14-fold water (mL/g)and 1.0 h each time. The optimal ethanol precipitation process was to concentrate the water extract to a relative density of 1.0 g/mL with alcohol content of liquid at 60% and precipitated for 12 h. Validation tests showed that RSDs of extract yield and beragenin content were all lower than 2%(n=3). The experiment of pharmacodynamics showed that water extract (before ethanol precipitation )and water extract after alcohol precipitation could significantly decrease the level of uric acid in hyperuricemia model mice (P<0.01). There was no statistical significance in the reduction of uric acid between 2 groups(P>0.05). CONCLUSIONS :The optimized water extraction technology can obtain good extract yield and bergenin content ,and combined with ethanol precipitation technology for removing excess impurities would not affect the pharmacodynamics. The water extraction and ethanol precipitation technology is feasible,and can be used for extracting the medicinal materials of Baijin granules and its edulcoration.
期刊: 2020年第31卷第08期
作者: 张雅,王一乔,马卓雅,梅之南,滕红丽
AUTHORS: ZHANG Ya,WANG Yiqiao ,MA Zhuoya ,MEI Zhinan ,TENG Hongli
关键字: 壮药;柏金颗粒;水提取;醇沉;正交试验;药效学;岩白菜素;高尿酸血症
KEYWORDS: Zhuang medicine ;Baijin granules ;Water extraction ;Ethanol precipitation ;Orthogonal test ;Pharmacodynamics;
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