伊贝母多糖的提取工艺优化及结构表征
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篇名: 伊贝母多糖的提取工艺优化及结构表征
TITLE: Study on Extraction Technology Optimization and Structural Characterization and of Fritillaria pallidiflora Poly- saccharide
摘要: 目的:优化伊贝母多糖(FPSP)的提取工艺,并对其结构进行研究。方法:采用Box-Behnken设计-响应面法,以FPSP产率为响应值,对FPSP提取工艺中的料液比、提取温度、提取时间进行优化。通过紫外吸收光谱法、傅里叶红外光谱(FTIR)法、气相色谱-质谱联用(GC-MS)法、刚果红染色法、扫描电子显微镜(SEM)观察、X射线衍射(XRD)法及热化学分析等表征手段对FPSP进行结构初探。结果:FPSP提取的最优工艺参数为料液比1∶28(g/mL)、提取温度94℃、提取时间2.5h,产率为16.25%(n=3),与理论产率(16.58%)的误差为0.33%。FPSP是由物质的量之比为1∶58.02∶0.73的木糖、葡萄糖、半乳糖及微量甘露糖组成,在280nm波长附近有弱吸收峰,属于α-构型的吡喃多糖,存在三螺旋结构,其表面呈显不规则颗粒堆积而成的网状结构,同时存在晶体与非晶体结构,具有较好的热稳定性。结论:优化后的FPSP提取工艺合理、产率较高,可为伊贝母资源的进一步开发利用提供参考。
ABSTRACT: OBJECTIVE:To optimize the extractio n technology of Fritillaria pallidiflora polysaccharides(FPSP),and to study its structure. METHODS :Using the yield of FPSP as response value ,Box-Behnken design-response surface methodology was adopted to optimize solid-liquid ratio ,extraction temperature and extraction time of FPSP extraction technology. Structural properties of FPSP was characterized by UV spectrum ,FTIR,GC-MS,Congo red staining ,SEM,XRD and thermogravimetric analysis. RESULTS:The optimal technology parameters of FPSP were solid-liquid ratio of 1∶28(g/mL),extraction temperature of 94 ℃, extraction time of 2.5 h;the yield of FPSP was 16.25%(n=3),the error of which to theoretical yield (16.58%)was 0.33%. FPSP contained xylose ,glucose and galactose with a molar ratio of 1∶58.02∶0.73,and trace amount of mannose ;there was a weak absorption peak near the wavelength of 280 nm;belonged to α-configuration pyranopolysaccharide. FPSP was in triple-helical structure. The surface of FPSP was a network structure formed by irregular particles. FPSP had both crystalline and amorphous structures. FPSP had good thermostability. CONCLUSIONS :The optimized extraction technology of FPSP is reasonable ,and has high extraction yield. The research might provide reference for the further development and utilization of F. pallidiflora .
期刊: 2020年第31卷第03期
作者: 阿衣吐逊·阿布都外力,帕尔哈提·柔孜,高彦华,排合尔丁·木台力甫,热合巴提·努尔夏提,宁慧霞,阿布力米提·伊力
AUTHORS: Ayituxun·Abuduwaili, Paerhati·Rouzi,GAO Yanhua,Paiheerding·Mutailifu,Rehebati·Nuerxiati,NING Huixia,Abulimiti·Yili
关键字: 伊贝母多糖;Box-Behnken设计;响应面法;结构表征;提取工艺
KEYWORDS: Fritillaria pallidiflora polysaccharide ; Box-Behnken design ; Response surface methodology ; Structural
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