苯扎贝特和非诺贝特对肺腺癌PC-9细胞增殖和c-myc表达的影响
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篇名: 苯扎贝特和非诺贝特对肺腺癌PC-9细胞增殖和c-myc表达的影响
TITLE:
摘要: 目的:研究贝特类药物苯扎贝特和非诺贝特对肺腺癌PC-9细胞增殖和c-myc表达的影响。方法:采用CCK8法检测苯扎贝特和非诺贝特(12.5、25、50、100、200 μmol/L)作用48 h对PC-9细胞存活率的影响。另将PC-9细胞分为给药组和对照组,给药组分别加入低、中、高浓度(25、50、100 μmol/L)的苯扎贝特和非诺贝特,对照组加入二甲基亚砜,作用48 h后,采用流式细胞仪检测细胞周期分布及凋亡情况;荧光定量聚合酶链法(qRT-PCR)检测细胞中c-myc mRNA相对表达量;Western blot法检测细胞中c-myc蛋白相对表达量。结果:上述浓度苯扎贝特作用下PC-9细胞存活率分别为(80.76±3.2)%、(74.35±5.06)%、(62.8±1.23)%、(59.03±1.55)%、(39.8±1.01)%;而非诺贝特作用下PC-9细胞存活率分别为(74.46±1.30)%、(61.91±4.77)%、(48.95±2.8)%、(37.05±1.55)%、(32.49±1.36)%。与对照组比较,苯扎贝特和非诺贝特的中、高浓度组G1期细胞比率均明显增加,苯扎贝特和非诺贝特的低、中、高浓度组的细胞凋亡率均明显增加,c-myc mRNA和蛋白相对表达量均明显降低,差异具有统计学意义(P<0.05)。结论:苯扎贝特和非诺贝特可抑制PC-9细胞增殖,并下调c-myc表达。
ABSTRACT: OBJECTIVE: To study the effects of bezafibrate (BEZ) and fenofibrate (FEN) on the proliferation of lung adenocarcinoma PC-9 cells and the expression of c-myc. METHODS: The effects of BEZ and FEN (12.5, 25, 50, 100, 200       μmol/L) on the survival rate of PC-9 cells were detected by CCK8 method. PC-9 cells were divided into administration group and control group. Administration group was given low, medium and high concentration (25, 50, 100 μmol/L) of BEZ and FEN; control group was treated with dimethyl sulfoxide for 48 h. Cell cycle distribution and apoptosis were detected by flow cytometry. qRT-PCR was used to detect mRNA relative expression of c-myc in cells. The protein relative expression of c-myc in cells were detected by Western blot assay. RESULTS: The survival rates of PC-9 cells were (80.76±3.2)%, (74.35±5.06)%, (62.8±1.23)%, (59.03±1.55)%, (39.8±1.01)% under the action of above concentration of BEZ; and the survival rates of PC-9 cells were (74.46±1.30)%, (61.91±4.77)%, (48.95±2.8)%, (37.05±1.55)%, (32.49±1.36)% under the action of FEN. Compared with control group, G1 phase cell ratio increased significantly in medium and high concentration groups of BEZ and FEN; the apoptotic rate of PC-9 cells was increased significantly in low, medium and high concentration groups of BEZ and FEN; mRNA and protein relative expression of c-myc were decreased significantly, with statistical significance (P<0.05). CONCLUSIONS: BEZ and FEN can inhibit the proliferation of PC-9 cells, and down-regulate c-myc expression.
期刊: 2019年第30卷第21期
作者: 王桂平,梁杰聪,李智斌,张彦焘
AUTHORS: WANG Guiping,LIANG Jiecong,LI Zhibin,ZHANG Yantao
关键字: 苯扎贝特;非诺贝特;肺腺癌PC-9细胞;半数抑制浓度;c-myc;抑制作用
KEYWORDS: Bezafibrate; Fenofibrate; Lung adenocarcinoma PC-9 cell; Half inhibitory concentration; c-myc; Inhibitory effect
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