HPLC法同时测定半枝莲饮片中4种黄酮类成分的含量及主成分分析
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篇名: HPLC法同时测定半枝莲饮片中4种黄酮类成分的含量及主成分分析
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摘要: 目的:建立同时测定半枝莲饮片中野黄芩苷、野黄芩素、木犀草素、芹菜素等4种黄酮类成分含量的方法,并进行主成分分析。方法:采用高效液相色谱法。色谱柱为Agilent ZOXDB-C18,流动相为甲醇-乙腈(80 ∶ 20,V/V)-1%醋酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为335 nm,柱温为30 ℃,进样量为10 μL。采用SPSS 20.0、SIMCA-P 13.0软件进行主成分分析。结果:野黄芩苷、野黄芩素、木犀草素、芹菜素进样量的线性范围分别为0.131~1.446 μg(r=0.999 0)、0.031~0.345 μg(r=0.999 7)、0.005~0.055 μg(r=0.999 2)、0.024~0.268 μg(r=0.999 2);定量限分别为1.178 8、0.602 9、0.744 1、1.079 1 ng,检测限分别为0.353 6、0.106 1、0.223 2、0.323 7 ng;精密度、稳定性、重复性试验的RSD均小于2%;加样回收率分别为99.38%~100.56%(RSD=0.44%,n=6)、91.01%~96.81%(RSD=2.43%,n=6)、91.44%~97.34%(RSD=2.59%,n=6)、96.21%~99.26%(RSD=1.23%,n=6)。主成分分析结果显示,主成分1和主成分2是影响样品质量评价的主要因子,2个主成分的累积方差贡献率为92.573%(>80%);S14-3样品综合评分最高,整体质量相对较好,S14-2、S14-1次之,这3批样品均系半枝莲种植基地药材加工产品,质量稳定。结论:本方法简单、快速,可用于同时测定半枝莲饮片中4种黄酮类成分的含量;主成分分析可为半枝莲饮片的质量控制提供参考。
ABSTRACT: OBJECTIVE: To establish the method for simultaneous determination of 4 kinds of flavones such as sutellarin, sutellarein, luteolin and apigenin in Scutellaria barbata decoction pieces, and to conduct principle component analysis. METHODS: HPLC method was adopted. The determination was performed on Agilent ZOXDB-C18 column with mobile phase consisted of methanol-acetonitrile (80 ∶ 20,V/V)-1% acetic acid solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 335 nm, and column temperature was 30 ℃. The sample size was 10 μL. Principal component analysis was carried out by SPSS 20.0 and SIMCA-P 13.0 software. RESULTS: The linear ranges of sutellarin, sutellarein, luteolin and apigenin were 0.131-1.446 μg(r=0.999 0), 0.031-0.345 μg(r=0.999 7), 0.005-0.055 μg(r=0.999 2), 0.024-0.268 μg(r=0.999 2), respectively. The limits of quantitation were 1.178 8, 0.602 9, 0.744 1, 1.079 1 ng; the limits of detection were 0.353 6, 0.106 1, 0.223 2, 0.323 7 ng;RSDs of precision, stability and reproducibility tests were all lower than 2%. The recoveries were 99.38%-100.56%(RSD=0.44%,n=6), 91.01%-96.81%(RSD=2.43%, n=6), 91.44%-97.34%(RSD=2.59%, n=6), 96.21%- 99.26%(RSD=1.23%,n=6), respectively. By principal component analysis, principal component 1 and prinicipal component 2 were main influential factors of sample, quality accumulative variance contribution rate of them was 92.573%(>80%). The comprehensive score of sample S14-3 was the highest, and the overall quality was relatively good; samples S14-2, S14-3 were the second. These 3 batches of sample were processed and produced in S. barbata planting base with stable quality. CONCLUSIONS: Established method is simple and rapid, and can be used for simultaneous determination of 4 kinds of flavones in S. barbata decoction pieces. Principle component analysis can provide reference for the quality control of S. barbata decoction pieces.
期刊: 2019年第30卷第20期
作者: 夏云岭,张振凌,张洪坤,林钦贤,梁伟龙,路丽
AUTHORS: XIA Yunling,ZHANG Zhenling,ZHANG Hongkun,LIN Qinxian,LIANG Weilong,LU Li
关键字: 半枝莲;野黄芩苷;野黄芩素;木犀草素;芹菜素;高效液相色谱法;含量测定;主成分分析
KEYWORDS: Scutellaria barbata; Sutellarin; Sutellarein; Luteolin; Apigenin; HPLC; Content determination; Principle component analysis
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