基于聚类分析和主成分分析的壮瑶药三妹木HPLC指纹图谱研究
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篇名: 基于聚类分析和主成分分析的壮瑶药三妹木HPLC指纹图谱研究
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摘要: 目的:建立壮瑶药三妹木的高效液相色谱(HPLC)指纹图谱,为不同产地三妹木的质量评价提供依据。方法:以广西南宁、桂林、梧州等5不同产地的10批三妹木为样品,采用“中药色谱指纹图谱相似度评价系统”(2012版)软件建立HPLC指纹图谱并进行相似度评价[色谱柱为Inertsil ODS-3,流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为0.8 mL/min,检测波长为350 nm,柱温为35 ℃,进样量为10 μL],通过对照品比对对共有峰进行指认。采用IBM SPSS 21.0软件对其进行聚类分析和主成分分析。结果:建立了HPLC指纹图谱,共确定了12个共有峰,并指认了其中3个共有峰(8、10、11号共有峰分别为夏佛塔苷、牡荆素和异牡荆素);10批样品的相似度均大于0.9。经聚类分析发现,10批药材可聚为二大类;经主成分分析发现,2个主成分因子的累计方差贡献率为86.108%(第一主成分、第二主成分的贡献率分别为66.891%、19.217%)。结论:成功建立了三妹木药材的HPLC指纹图谱,该方法简单、易行,为三妹木的质量控制提供了可靠的评价方法。
ABSTRACT: OBJECTIVE: To establish HPLC fingerprint of Zhuang and Yao medicine Lespedeza formosa, and to provide reference for quality control of L. formosa from different producing areas. METHODS: Totally 10 batches of samples were collected from 5 producing areas as Guangxi Nanning, Guilin, Wuzhou and so on. HPLC fingerprints was established and similarity analysis was carried out by using “Similarity evaluation system of TCM chromatographic fingerprint” (2012 edition) software. The determination was performed on Inertsil ODS-3 column with mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL/min. The detection wavelength was set at 350 nm, and the column temperature was 35 ℃. The sample size was 10 μL. Common peaks were identified by comparing substance control. Cluster analysis and principal compoent analysis were performed by using IBM SPSS 21.0 statistical software. RESULTS: HPLC fingerprint was established, and 12 common peaks were calibrated. 3 common peaks were identified (common peak 8, 10, 11 were chafotalin, vitexin and isovitexin). The similarity of 10 batches of samples were all higher than 0.9. Through cluster analysis, 10 batches of medicinal materials could be clutered into 2 groups. According to the principal component analysis, the cumulative variance contribution rate of the two principal component factors was 86.108% (contribution rates of first principal components and second principal components were 66.891% and 19.217%). CONCLUSIONS: HPLC fingerprint of L. formosa is established successfully. The method is simple and easy to use, provides a reliable evalution method for quality control of L. formosa.
期刊: 2019年第30卷第17期
作者: 傅静,张莹,李宇辉,王孝勋
AUTHORS: FU Jing,ZHANG Ying,LI Yuhui,WANG Xiaoxun
关键字: 壮瑶药;三妹木;高效液相色谱法;化学计量学;指纹图谱
KEYWORDS: Zhuang and Yao medicine; Lespedeza formosa; HPLC; Chemometrics; Fingerprint
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