HPLC法同时测定辣木叶颗粒中异槲皮苷、紫云英苷和丹酚酸B的含量
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篇名: HPLC法同时测定辣木叶颗粒中异槲皮苷、紫云英苷和丹酚酸B的含量
TITLE:
摘要: 目的:建立同时测定辣木叶颗粒中异槲皮苷、紫云英苷和丹酚酸B含量的方法。方法:采用高效液相色谱(HPLC)法。色谱柱为Cosmosil-C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.3 mL/min,柱温为40 ℃,检测波长为260 nm,进样量为10 μL。结果:异槲皮苷、紫云英苷和丹酚酸B检测质量浓度线性范围分别为0.017~0.341、0.010~0.194、0.010~0.195 mg/mL(r均>0.999 0);检测限分别为0.085、0.143、0.117 μg/mL;定量限分别为0.283、0.476、0.392 μg/mL;精密度、稳定性(24 h)、重复性试验的RSD均≤2.0%(n=6);平均加样回收率分别为101.22%、98.76%、98.72%,RSD分别为0.66%、0.30%、0.30%(n=6)。结论:建立的方法简便、准确、重复性好, 可用于同时测定辣木叶颗粒中异槲皮苷、紫云英苷和丹酚酸B的含量。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of isoquercitrin, astragalin and salvianolic acid B in Moringa oleifera leaves granules. METHODS: HPLC method was adopted. The determination was performed on Cosmosil-C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (gradient elution) at flow rate of 1.3 mL/min. The column temperature was 40 ℃ and detection wavelength was set at 260 nm. The sample size was 10 μL. RESULTS: The linear ranges of isoquercitrin, astragalin and salvianolic acid B were 0.017-0.341, 0.010-0.194, 0.010-0.195 mg/mL, respectively (all r>0.999 0). The detection limits were 0.085, 0.143, 0.117 μg/mL, and the limits of quantitation were 0.283, 0.476, 0.392 μg/mL, respectively. RSDs of precision, stability (24 h) and reproducibility tests were all lower than 2.0% (n=6). Average recoveries were 101.22%, 98.76% and 98.72%, and RSDs were 0.66%,0.30%,0.30% (n=6), respectively. CONCLUSIONS: The established method is simple, accurate and reproducible. It can be used for simultaneous determination of isoquercitrin, astragalin and salvianolic acid B in M. oleifera leaves granules.
期刊: 2019年第30卷第9期
作者: 杨海洋,刘洋,李梦薇,李雪岩,何军,顾文宏,尹济云
AUTHORS: YANG Haiyang,LIU Yang,LI Mengwei,LI Xueyan,HE Jun,GU Wenhong,YIN Jiyun
关键字: 辣木叶颗粒;高效液相色谱法;异槲皮苷;紫云英苷;丹酚酸B;含量测定
KEYWORDS: Moringa oleifera leaves granules; HPLC; Isoquercitrin; Astragalin; Salvianolic acid B; Content determination
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