吉产不同生长年限人参中8种主要人参皂苷与人参皂苷Rg1比值的变化规律研究
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篇名: 吉产不同生长年限人参中8种主要人参皂苷与人参皂苷Rg1比值的变化规律研究
TITLE:
摘要: 目的:探讨吉产不同生产年限人参中8种主要人参皂苷与人参皂苷Rg1的比值的变化规律,为其生长年限的鉴别提供参考。方法:收集吉林省不同生长年限(3~30年)的园参、林下参、野山参样品,采用高效液相色谱法(HPLC)测定人参中人参皂苷Rg1、Re、Rf、Rb1、Rc、Rb2、Rb3、Rd 等8种成分的含量;计算各皂苷单体含量及8种人参皂苷总含量分别与人参皂苷Rg1含量的比值,并探究该比值与生长年限的关系。结果:随人参生长年限的增长,园参中8种人参皂苷总含量与人参皂苷Rg1的比值及人参皂苷Re、Rb1、Rc、Rd单体含量与人参皂苷Rg1的比值均逐渐减小(P<0.001),林下参中人参皂苷Re与人参皂苷Rg1的比值呈先减小后增加趋势(P<0.001),野山参中8种人参皂苷总含量与人参皂苷Rg1的比值及人参皂苷Re、Rb1与人参皂苷Rg1的比值均逐渐增加(P<0.001),而园参、林下参、野山参中人参皂苷Rf、Rb3与人参皂苷Rg1的比值则均无明显差异(P>0.05)。结论:园参、林下参、野山参均含有8种人参皂苷成分,可根据人参皂苷Rg1含量和人参皂苷Re、Rb1单体含量与人参皂苷Rg1的比值初步推测其生长年限。
ABSTRACT: OBJECTIVE: To investigate the proportion of 8 kinds of ginsenoside to ginsenoside Rg1 in Panax ginseng and the regularity of growth year in Jilin province, and to provide reference for the identification of growth year. METHODS: The samples of garden ginseng, wild-cultivated ginseng and wild ginseng were collected from different growth years (3-30 years) in Jilin province. The contents of 8 ginsenoside (ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd) in P. ginseng were determined by HPLC. The contents of saponins as well as the proportion of 8 kinds of ginsenoside to ginsenoside Rg1 were calculated; the relationship of the proportion with growth year was investigated. RESULTS: As the increase of growth year, the proportion of 8 kinds of ginsenosides in garden ginseng to ginsenoside Rg1 as well as that of ginsenoside Re, Rb1, Rc, Rd to ginsenoside Rg1 were decreased gradally (P<0.001); the proportion of ginsenoside Re to ginsenoside Rg1 in wild-cultivated ginseng decreased first and then increased(P<0.001); the proportion of 8 kinds of ginsenosides to ginsenoside Rg1 as well as the proportion of ginsenoside Re and Rb1 to ginsenoside Rg1 were increased gradually in wild ginseng (P<0.001); the proportion of ginsenoside Rf, Rb3 to ginsenoside Rg1 in garden ginseng, wild-cultivated ginseng and wild ginseng had no significant difference(P>0.05). CONCLUSIONS: Garden ginseng, wild-cultivated ginseng and wild ginseng contain 8 kinds of ginsenosides. The growth year can be predicted preliminarily according to the proportion of ginsenoside Rg1 and ginsenoside Re, Rb1 to ginsenoside Rg1.
期刊: 2019年第30卷第1期
作者: 余江锋,李育平,何伟,杨剑湖,贝伟剑,郭姣
AUTHORS: YU Jiangfeng,LI Yuping,HE Wei,YANG Jianhu,BEI Weijian,GUO Jiao
关键字: 吉林省;人参;生长年限;人参皂苷
KEYWORDS: Jilin province; Panax ginseng; Growth years; Ginsenoside
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