白及药材的HPLC指纹图谱建立及聚类分析
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篇名: 白及药材的HPLC指纹图谱建立及聚类分析
TITLE:
摘要: 目的:建立白及药材的高效液相色谱(HPLC)指纹图谱,并对样品进行聚类。方法:采用HPLC法,色谱柱为Diamonsil C18,流动相为0.1%磷酸溶液-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为270 nm,柱温为30 ℃,进样量为10 μL。以1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯为参照,绘制10批药材样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行相似度评价,确定共有峰,并采用SPSS 23.0统计软件对其进行聚类分析。结果:10批药材样品的HPLC图谱有15个共有峰,其中9批相似度均大于0.90;经验证,10批药材样品HPLC图谱与对照指纹图谱具有较好的一致性。10批药材样品可聚为3类,S1、S2、S3、S4、S6、S8、S9、S10聚为第1类,S7聚为第2类,S5为第3类。结论:所建HPLC指纹图谱和聚类分析结果可为白及药材的质量控制提供参考。
ABSTRACT: OBJECTIVE: To establish HPLC fingerprint of Bletilla striata, and conduct cluster analysis. METHODS: HPLC method was adopted. The determination was performed on Diamonsil C18 column with mobile phase consisted of 0.1% phosphoric acid solution-acetonitrile (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm, and column temperature was 30 ℃. The sample size was 10 μL. Using 1, 4-two [4-(glucose oxy) benzyl]-2-isobutyl malate as reference, HPLC chromatograms of 10 batches of medicinal samples were determined. Similarity evaluation was conducted by using Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A edition). Common peaks were determined, and cluster analysis was also conducted for it by using SPSS 23.0 statistic software. RESULTS: There were 15 common peaks in 10 batches of samples, and the similarities of 9 batches were more than 0.90. After validation, HPLC chromatograms of 10 batches of medicinal material were all in agreement with control fingerprint. The 10 batches of samples could be clustered into 3 categories. S1, S2, S3, S4, S6, S8, S9, S10 were clustered into category 1; S7 was clustered into category 2; S5 was clustered into category 3. CONCLUSIONS: Established fingerprint and the cluster analysis results can provide reference for the quality control of B. striata.
期刊: 2018年第29卷第22期
作者: 刘刚,丁志山,刘育辰,张永萍,万超,罗成凯
AUTHORS: LIU Gang,DING Zhishan,LIU Yuchen,ZHANG Yongping,WAN Chao,LUO Chengkai
关键字: 白及;高效液相色谱法;1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯;指纹图谱;聚类分析
KEYWORDS: Bletilla striata; HPLC; 1, 4-two [4-(glucose oxy) benzyl]-2-isobutyl malate; Fingerprint; Cluster analysis
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