续断饮片标准汤中川续断皂苷Ⅵ转移率的研究
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篇名: 续断饮片标准汤中川续断皂苷Ⅵ转移率的研究
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摘要: 目的:研究续断饮片标准汤中川续断皂苷Ⅵ的转移率。方法:采用高效液相色谱法测定续断饮片及其标准汤中川续断皂苷Ⅵ的含量,色谱柱为SinoChrom ODS-AP,流动相为乙腈-水(30 ∶ 70,V/V),流速为1.0 mL/min,检测波长为212 nm,柱温为30 ℃,进样量为10 μL。根据成分含量测定结果计算从饮片到标准汤转变过程中川续断皂苷Ⅵ的转移率。结果:川续断皂苷Ⅵ检测进样量线性范围为0.484~4.84 μg(r=0.999 9);精密度、稳定性、重复性试验的RSD均小于2%;定量限、检测限分别为0.3、0.1 μg;饮片和标准汤中的加样回收率分别为95.13%~100.22%(RSD=1.78%,n=6)、97.07%~100.08%(RSD=0.98%,n=6);耐用性试验的RSD均小于1%。续断饮片标准汤中川续断皂苷Ⅵ的转移率为26.3%~49.5%。结论:该方法操作简便、准确,精密度、稳定性、重复性、耐用性好,可用于续断饮片标准汤中川续断皂苷Ⅵ转移率的测定。
ABSTRACT: OBJECTIVE: To study transfer rate of asperosaponinⅥ in standard decoction of Dipsacus asper decoction pieces. METHODS: The content of asperosaponin Ⅵ in Dipsacus asper decoction pieces and its standard decoction was determined by HPLC. The determination was performed on SinoChrom ODS-AP with mobile phase consisted of acetonitrile-water (30 ∶ 70,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 212 nm, and column temperature was 30 ℃. The sample size was 10 μL. By the ingredients content obtained, the transfer rate of asperosaponin Ⅵ was calculated during decoction piece to standard decoction. RESULTS: The linear range of asperosaponin Ⅵ was 0.484-4.84 μg(r=0.999 9). RSDs of precision, stability and reproducibility tests were all lower than 2%. The limits of quantification and detection were 0.3 and 0.1 μg, respectively. The average recoveries in D. asper decoction pieces and standard decoction were 95.13%-100.22%(RSD=1.78%,n=6),97.07%-100.08%(RSD=0.98%,n=6). RSD of durability test was lower than 1%.The transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces ranged 26.3%-49.5%. CONCLUSIONS: The method is simple, accurate, precise, stable, reproducible and durable, and can be used for transfer rate of asperosaponin Ⅵ in standard decoction of D. asper decoction pieces.
期刊: 2018年第29卷第20期
作者: 李军山,常云凤,马浩,牛丽颖,李振江
AUTHORS: LI Junshan,CHANG Yunfeng,MA Hao,NIU Liying,LI Zhenjiang
关键字: 续断;饮片;标准汤;高效液相色谱法;川续断皂苷Ⅵ;转移率
KEYWORDS: Dipsacus asper; Decoction pieces; Standard decoction; HPLC; Asperosaponin Ⅵ; Transfer rate
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