丹红注射液对急性心肌梗死模型大鼠炎症反应和心肌形态学的影响
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篇名: 丹红注射液对急性心肌梗死模型大鼠炎症反应和心肌形态学的影响
TITLE:
摘要: 目的:研究丹红注射液(DHI)对急性心肌梗死(简称“心梗”)模型大鼠心肌保护的作用机制。方法:将30只大鼠按照随机数字表法分成假手术组(生理盐水,肌内注射)、模型组(生理盐水,肌内注射)和DHI组(0.76 mL/kg,肌内注射),每组10只。除假手术组外,其余各组大鼠均采用左冠状动脉前降支结扎法复制心梗模型。造模成功后,各组大鼠每天给药1次,连续给药14 d。在术后24、48 h,采用全自动生化分析仪检测各组大鼠血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)和肌酸激酶同工酶(CK-MB)水平;给药14 d后,采用酶联免疫吸附法检测各组大鼠血清中白细胞介素1β(IL-1β)、C反应蛋白(CRP)和肿瘤坏死因子α(TNF-α)水平,采用超声仪观察各组大鼠左心室室壁运动变化情况,并结合苏木精-伊红染色及MASSON染色进行心肌形态学分析。结果:与假手术组比较,模型组大鼠在术后24 h血清中LDH、CK、CK-MB水平显著升高(P<0.05);给药14 d后血清中IL-1β、CRP、TNF-α水平显著升高(P<0.05),左心室前壁几乎无波动,且心肌组织中仅有少量心肌细胞存活,出现细胞体积增大、排列松散,细胞核固缩或溶解等变化,心梗面积百分比显著升高(P<0.05)。与模型组比较,DHI组大鼠在术后48 h血清中LDH、CK、CK-MB水平显著降低(P<0.05);给药14 d后血清中IL-1β、CRP、TNF-α水平显著降低(P<0.05),左心室扩张程度明显减轻,心室壁增厚且出现波动,心肌纤维化程度降低,心肌细胞坏死数量减少,心梗面积百分比显著降低(P<0.05)。结论:DHI可通过降低心肌酶谱和炎症因子水平抑制心肌纤维化,从而改善心梗。
ABSTRACT: OBJECTIVE: To study the effects of Danhong injection (DHI) on myocardial protection of acute myocardial infarction (AMI) model rats. METHODS: Totally 30 rats were randomly divided into sham operation group (normal saline, im), model group (normal saline, im) and DHI group (0.76 mL/kg, im) according to random number table method, with 10 rats in each group. Except for sham operation group, AMI model was induced by ligation of left anterior descending coronary artery in other groups. After modeling, rats were given relevant medicine once a day, for consecutive 14 d. 24, 48 h after operation, the serum lsevels of LDH, CK and CK-MB were determined by automatic biochemical analyzer. 14 d after medication, the serum levels of IL-1β, CRP and TNF-α were determined by ELISA. Echocardiography was applied to observe the changes of left ventricular wall movement of rats in each group. Based on HE staining and MASSON staining, morphological analysis was performed for myocardial. RESULTS: Compared with sham operation group, the serum levels of LDH, CK and CK-MB in model group were increased significantly 24 h after operation (P<0.05). 14 d after medication, the serum levels of IL-1β, CRP and TNF-α were increased significantly (P<0.05); there was almost no fluctuation in the anterior wall of the left ventricle; in the myocardial tissue, only a small number of myocardial cells survived, while cell volume increased and arranged loosely; nuclear condensation or dissolution were also found; the percentage of myocardial infarction area was increased significantly (P<0.05). Compared with model group, the serum levels of LDH, CK and CK-MB in DHI group were decreased significantly 48 h after operation (P<0.05). Serum levels of IL-1β, CRP and TNF-α were decreased significantly 14 d after medication (P<0.05). The degree of left ventricle dilation decreased significantly, and ventricular wall thickened and fluctuated; myocardial fibrosis decreased, necrosis of myocardial cells decreased, and the percentage of myocardial infarction area decreased significantly (P<0.05). CONCLUSIONS: DHI plays the role of improvement of AMI by decreasing the levels of myocardial enzyme spectrum indexes and inflammatory factors and inhibiting myocardial fibrosis.
期刊: 2018年第29卷第19期
作者: 施洋,候宝林,樊登峰,樊官伟
AUTHORS: SHI Yang,HOU Baolin,FAN Dengfeng,FAN Guanwei
关键字: 丹红注射液;心肌梗死模型;心肌酶谱;炎症因子;形态学分析;大鼠
KEYWORDS: Danhong injection; Acute myocardial infarction model; Myocardial enzyme spectrum; Inflammatory factors; Morphological analysis; Rat
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