ICP-MS法同时测定林下西洋参和园地栽培西洋参中14种微量元素的含量
x

请在关注微信后,向客服人员索取文件

篇名: ICP-MS法同时测定林下西洋参和园地栽培西洋参中14种微量元素的含量
TITLE:
摘要: 目的:建立同时测定林下西洋参和园地栽培西洋参中14种微量元素含量的方法。方法:采用电感耦合等离子体质谱法。射频功率为1 350 W,采样深度为7.0 mm,载气流量为1.1 L/min,雾化室温度为2 ℃,蠕动泵转速分辨率为0.1 rps,等离子体气流量为15.0 L/min,雾化器为高盐雾化器,氧化物指标(氧化铈离子/铈)<0.5%,双电荷指标(二价钡离子/一价钡离子)<1%;微波消解功率为3 600 W,升温程序为190 ℃保持48 h,冷却至室温,再升温至150 ℃,保持12 h。结果:锰、铬、锶、锌、铷、硼、镍、铜、钒、锡、砷、钼、硒、钴元素检测质量浓度线性范围分别为0.1~500 μg/L(r=0.999 8)、0.5~500 μg/L(r=0.999 9)、0.1~500 μg/L(r=0.999 9)、0.5~500 μg/L(r=0.999 9)、0.5~500 μg/L(r=0.999 9)、0.1~200 μg/L(r=0.999 9)、0.5~500 μg/L(r=0.999 9)、1~500 μg/L(r=0.999 9)、0.1~500 μg/L(r=0.999 8)、0.1~200 μg/L(r=0.999 8)、0.5~500 μg/L(r=0.999 9)、0.1~200 μg/L(r=0.999 8)、0.5~500 μg/L(r=0.999 9)、0.1~500 μg/L(r=0.999 9);定量限均小于0.60 μg/L,检测限均小于0.20 μg/L;精密度、稳定性、重复性试验的RSD均小于3%;加样回收率为97.08%~106.65%(RSD为0.64%~2.33%,n=9);林下西洋参含量为0.288 2~148.600 0 μg/g,园地栽培西洋参含量为0.135 7~65.190 0 μg/g。结论:该方法灵敏度高、准确性好,可用于同时测定林下西洋参和园地栽培西洋参中14种微量元素的含量;林下西洋参和园地栽培西洋参中14种微量元素含量差异显著,总体上林下西洋参较园地栽培西洋参中微量元素的含量更高,表明两种生长环境对西洋参的成分影响较大。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of 14 kinds of microelements from underwood-cultivated Panax quinquefolius and garden-cultivated P. quinquefolius. METHODS: ICP-MS method was adopted. The radio frequency power was 1 350 W, and sampling depth was 7.0 mm; carrier gas velocity was 1.1 L/min, and atomizing chamber temperature was 2 ℃; peristaltic pump was 0.1 rps; plasma gas flow rate was 15.0 L/min in high-salt nebulizer as atomizer; oxide index(CeO+/Ce+) is less than 0.5% and the double charge index(Ba2+/Ba+) is less than 1%. The microware digestion power is 3 600 W, and the heating process is heated to 190 ℃, maintained at 48 h, and cooling to room temperature, and the heated 150 ℃, maintained 12 h.  RESULTS: The linear range of Mn, Cr, Sr, Zn, Rb, B, Ni, Cu, V, Sn, As, Mo, Se and Co were 0.1-500 μg/L(r=0.999 8),0.5-500 μg/L(r=0.999 9),0.1-500 μg/L(r=0.999 9),0.5-500 μg/L(r=0.999 9),0.5-500 μg/L(r=0.999 9),0.1-200 μg/L(r=0.999 9),0.5-500 μg/L(r=0.999 9),1-500 μg/L(r=0.999 9),0.1-500 μg/L(r=0.999 8),0.1-200 μg/L(r=0.999 8),0.5-500 μg/L(r=0.999 9),0.1-200 μg/L(r=0.999 8),0.5-500 μg/L(r=0.999 9) and 0.1-500 μg/L(r=0.999 9), respectively. The limits of quantitation were less than 0.60 μg/L, and the limits of detection were lower than 0.20 μg/L. RSDs of precision, stability and reproducibility tests were no more than 3%; the recoveries were 97.08%-106.65%(RSDs were 0.64%-2.33%,n=9); the content of underwood-cultivated P. quinquefolius were 0.288 2-148.600 0 μg/g, the content of garden-cultivated P. quinquefolius were 0.135 7-65.190 0 μg/g.  CONCLUSIONS: The method is sensitive, accurate and can be used for simultaneous determination of 14 kinds of microelements. There are great differences in 14 kinds of microelements between underwood-cultivated P. quinquefolius and garden-cultivated P. quinquefolius. On the whole, the contents of microelements in underwood-cultivated P. quinquefolius are higher than garden-cultivated P. quinquefolius. The cultivation environments have great influence on the growth of P. quinquefolius.
期刊: 2018年第29卷第16期
作者: 林红强,谭静,王涵,吴福林,董庆海,李平亚,刘金平
AUTHORS: LIN Hongqiang,TAN Jing,WANG Han,WU Fulin,DONG Qinghai,LI Pingya,LIU Jinping
关键字: 林下西洋参;园地栽培西洋参;电感耦合等离子体质谱法;微量元素;含量测定
KEYWORDS: Underwood-cultivated Panax quinquefolius; Garden-cultivated P. quinquefolius; ICP-MS; Microelements; Content determination
阅读数: 150 次
本月下载数: 5 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!