头花蓼内生真菌Aspergillus terreus油脂类代谢物的鉴定及其抗多药耐药菌和抗炎作用研究
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篇名: 头花蓼内生真菌Aspergillus terreus油脂类代谢物的鉴定及其抗多药耐药菌和抗炎作用研究
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摘要: 目的:鉴定头花蓼内生真菌Aspergillus terreus油脂类代谢物并考察其抗多药耐药菌和抗炎作用。方法:采用气相色谱-质谱联用技术(GC-MS)分析、鉴定A. terreus的油脂类代谢物;采用96孔板微量稀释法测试油脂类代谢物和主要成分对10株多药耐药菌(肺炎克雷伯杆菌、普通变形杆菌、表皮葡萄球菌、大肠埃希菌、金黄色葡萄球菌、阴沟肠杆菌、铜绿假单胞菌、奇异变形杆菌、屎肠球菌和鲍曼不动杆菌)的最低抑菌浓度(MIC),用涂布平板法测定各样品对细菌的最低杀菌浓度(MBC)。以脂多糖诱导体外RAW264.7细胞炎症模型,考察不同质量浓度(50、100、200 μg/mL)油脂类代谢物作用24 h后对细胞中一氧化氮(NO)、肿瘤坏死因子α(TNF-α)释放的影响。结果:从A. terreus的油脂类代谢物中共鉴定了13个化合物,其中棕榈酸、硬脂酸、亚油酸和油酸为主要成分,其相对百分含量依次为29.35%、10.87%、21.94%、34.85%。油脂类代谢物对大肠埃希菌和肺炎克雷伯杆菌表现出较好的抗菌活性,MIC分别为12.5、50 mg/mL,MBC分别为25、100 mg/mL;4个主要成分均具有抑制大肠埃希菌生长的作用,MIC范围为0.5~1 mg/mL,其中棕榈酸显示出较为广泛的抗菌活性,且对屎肠球菌活性最强(MIC为0.25 mg/mL)。50、100 μg/mL的油脂类代谢物可显著抑制RAW264.7细胞中炎症因子NO和TNF-α的释放。结论:头花蓼内生真菌A. terreus的油脂类代谢物具有抗多药耐药菌和抗炎作用。
ABSTRACT: OBJECTIVE: To identify lipid metabolites of endophytic fungus Aspergillus terreus from Polygonum capitatum, and to investigate their anti-multidrug resistant bacteria and anti-inflammatory effects. METHODS: GC-MS was used to analyze and identify lipid metabolites of A. terreus. MIC of lipid metabolites and main composition to 10 strains of multidrug resistant bacteria (Klebsiella pneumoniae, Proteus common, Staphylococcus epidermidis, Escherichia coli, Staphylococcus aureus, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Enterococcus faecium and Acinetobacter baumanii) were determined by 96-well plate microdilution method. The spread plate method was used to determine MBC of samples to bacteria. LPS-induced RAW264.7 inflammatory model was used to investigate the effects of different mass concentrations (50, 100, 200 μg/mL) of lipid metabolites on the release of NO and TNF-α after treated for 24 h. RESULTS: A total of 13 compounds were identified in lipid metabolites of A. terreus, among which palmitic acid, stearic acid, linoleic acid and oleic acid were main components, and relative percentages of them were 29.35%, 10.87%, 21.94%, 34.85%. The lipid metabolites displayed anti-bacterial activity against E. coli and K. pneumonia with MICs of 12.5 mg/mL and 50 mg/mL, MBC of 25 mg/mL and 100 mg/mL, respectively. The main 4 compositions could inhibit the growth of E. coli, with MIC of 0.5-1 mg/mL, among which palmitic acid showed significant antibacterial activity, especially to E. faecium (MIC of 0.25 mg/mL). 50, 100 μg/mL lipid metabolites could signifiantly inhibit the release of inflammatory factor of NO and TNF-α in RAW264.7 in vitro. CONCLUSIONS: The lipid metabolites of endophytic fungus A. terreus from P. capitatum show anti- multi-drug resistant bacteria and anti-inflammatory effects.
期刊: 2018年第29卷第11期
作者: 刘俊,张青艳,杨馨,周孟,廖尚高,徐国波
AUTHORS: LIU Jun,ZHANG Qingyan,YANG Xin,ZHOU Meng,LIAO Shanggao,XU Guobo
关键字: 头花蓼;内生真菌Aspergillus terreus;油脂类代谢物;抗多药耐药菌活性;抗炎作用
KEYWORDS: Polygonum capitatum; Endophytic fungus Aspergillus terreus; Lipid metabolites; Anti-multidrug resistant bacteria activity; Anti-inflammatory effect
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