金钗石斛叶中总黄酮的提取分离及体外抗阿尔茨海默病活性研究
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篇名: 金钗石斛叶中总黄酮的提取分离及体外抗阿尔茨海默病活性研究
TITLE:
摘要: 目的:对金钗石斛叶中总黄酮进行提取分离,并考察其体外抗阿尔茨海默病(AD)活性。方法:采用超声提取法提取金钗石斛叶中总黄酮,将所得浸膏用水分散后分别用氯仿、乙酸乙酯和正丁醇进行萃取,采用显色反应和薄层色谱法对各极性提取物进行定性分析,硝酸铝-亚硝酸钠法定量分析各提取物中总黄酮含量。采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除试验考察各提取物的体外抗氧化活性,硫黄素T法考察各提取物对Aβ42蛋白聚集的抑制作用,紫外-可见光谱扫描法测定各提取物对金属离子(Cu2+,Fe3+和Zn2+)的螯合能力,以考察其体外抗AD活性。结果:乙酸乙酯、正丁醇、水提取物中均含有黄酮成分,含量分别为0.03、0.12、0.05 mg/mL。3种提取液对DPPH自由基的半数清除浓度(IC50)分别为0.021、0.011、0.013 mg/mL,对Aβ42蛋白聚集的抑制率分别为43.77%、52.28%、38.42%,对金属离子具有一定的螯合能力,其中尤以对Cu2+的螯合作用最为明显。结论:金钗石斛叶中总黄酮具有较好的抗氧化、抑制Aβ42蛋白聚集及螯合金属离子的能力,具有一定的体外抗AD活性,且以正丁醇提取物效果最明显。
ABSTRACT: OBJECTIVE: To separate and isolate total flavonoids from Dendrobium nobile leaves, and to investigate its anti-Alzheimer’s disease (AD) activity in vitro. METHODS: Total flavonoids were obtained by ultrasonic extraction method and extracted by chloroform, ethyl acetate and butyl alcohol after the obtained extract was dispersed with water. Qualitative analysis was carried out with color reaction and TLC. The content of total flavonoids in extracts was analyzed quantitatively by Aluminum nitrate-sodium nitrite method. Antioxidant activity of extract was investigated by DPPH radical scavenging assay; the inhibitory effect of each extract on Aβ42 protein aggregation was investigated by Thioflavin T assay. Metals (Cu2+, Fe3+, Zn2+) chelating property was studied by UV-vis spectrum scanning to investigate the anti-AD activity in vitro. RESULTS: The flavonoids were found in ethyl acetate, butyl alcohol and aqueous extracts, and their flavonoids contents were 0.03, 0.12, 0.05 mg/mL, respectively. IC50 of three extracts to DPPH free radicals were 0.021, 0.011, 0.013 mg/mL. Inhibitory rates of them to Aβ42 protein aggregation were 43.77%, 52.28%, 38.42%, respectively. Three extracts exerted metal chelating ability which was best in Cu2+. CONCLUSIONS: The total flavonoids from D. nobile leaves have good antioxidant activities, Aβ42 aggregation inhibitory activities and metal chelating activity, show certain anti-AD activity in vitro especially in butyl alcohol extract.
期刊: 2018年第29卷第3期
作者: 李艳萍,李海燕,纪晓婉,岑志芳,颜金武,吴剑峰
AUTHORS: LI Yanping,LI Haiyan,JI Xiaowan,CEN Zhifang,YAN Jinwu,WU Jianfeng
关键字: 金钗石斛叶;黄酮成分;阿尔茨海默病;抗氧化;Aβ42蛋白
KEYWORDS: Dendrobium nobile leaves; Flavonoids; Alzheimer’s disease; Antioxidant; Aβ42 protein
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