声光可调-近红外漫反射光谱法快速评价黄芪药材质量
x

请在关注微信后,向客服人员索取文件

篇名: 声光可调-近红外漫反射光谱法快速评价黄芪药材质量
TITLE:
摘要: 目的:建立快速评价黄芪药材质量的方法。方法:采用烘干法测定黄芪药材样品水分含量,采用高效液相色谱(HPLC)-蒸发光散射检测法(ELSD)测定黄芪甲苷的含量,采用HPLC法测定毛蕊异黄酮葡萄糖苷的含量(均作为参考值)。采用声光可调-近红外漫反射光谱法结合偏最小二乘法建立预测黄芪药材中上述指标含量的定量模型(作为预测值)。根据参考值,采集60批药材样品,采用一阶导数联合平滑降噪法预处理光谱,水分、黄芪甲苷、毛蕊异黄酮葡萄糖苷含量预测的最佳波段分别为1 100~2 300、1 080~2 160、1 170~2 230 nm。结果:药材样品中水分、黄芪甲苷、毛蕊异黄酮葡萄糖苷的含量测定方法学考察符合要求。水分、黄芪甲苷、毛蕊异黄酮葡萄糖苷定量模型校正均方根偏差分别为0.132 3、0.006 6、0.002 5,预测均方根偏差分别为0.237 1、0.016 3、0.004 7,校正集内部交叉验证相关系数分别为0.975 9、0.953 3、0.968 0;定量模型内部验证偏差分别为1.43%、1.90%、1.84%,外部验证偏差分别为1.73%、2.68%、2.71%。结论:该方法快速、准确、简便、无污染,可用于黄芪药材质量的快速评价。
ABSTRACT: OBJECTIVE: To establish the method for rapid quality evaluation of Astragali Radix. METHODS: The moisture of medicinal material was determined by oven drying method; the content of astragaloside Ⅳ was determined by HPLC-ELSD; the content of isoflavone glucoside was determined by HPLC (as reference value). The partial least squares (PLS) method combined with acousto-optic turnable filter-NIDRS was adopted to build quantitative model of above indexes in Astragali Radix (as predict value). According to reference value, 60 batches of sample were collected. The spectra pretreatment was conducted by first derivative method combined with Savitzky golay. The optimal bands of moisture, astragaloside Ⅳ and isoflavone glucoside were 1 100- 2 300 nm,1 080-2 160 nm,1 170-2 230 nm,respectively. RESULTS: The content determination of moisture, astragaloside Ⅳ and isoflavone glucoside in samples were all in line with methodology requirements. The corrected mean square root deviation of quantitative model for moisture, astragaloside Ⅳ, calycosin glucoside were 0.132 3, 0.006 6, 0.002 5, respectively; predicted mean square root deviation were 0.237 1, 0.016 3, 0.004 7; internal cross validation coefficient of correction set were 0.975 9, 0.953 3, 0.968 0; internal verification deviation of quantitative model were 1.43%, 1.90%, 1.84%; external verification deviation were 1.73%, 2.68%, 2.71%, respectively. CONCLUSIONS: The method is rapid, accurate,simple,pollution-free, and can be used for rapid quality evaluation of Astragali Radix.
期刊: 2018年第29卷第2期
作者: 任绪华,苏婷,姜文月,李亚东,郑广晶,高陆
AUTHORS: REN Xuhua,SU Ting,JIANG Wenyue,LI Yadong,ZHENG Guangjing,GAO Lu
关键字: 近红外漫反射光谱法;声光可调;黄芪;水分;黄芪甲苷;毛蕊异黄酮葡萄糖苷
KEYWORDS: NIDRS; Acousto-optic turnable filter; Astragali Radix; Moisture; Astragaloside Ⅳ; Isoflavone glucoside
阅读数: 171 次
本月下载数: 20 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!