合江金钗石斛在不同采收期时多糖和石斛碱含量的比较
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篇名: 合江金钗石斛在不同采收期时多糖和石斛碱含量的比较
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摘要: 目的:对不同采收年限与采收月份时合江金钗石斛的品质指标进行测定,为合江金钗石斛合理采收期的确定提供科学依据。方法:采收一年生、二年生和三年生金钗石斛茎,测定其折干率和多糖(比色法)、石斛碱(气相色谱法)、总生物碱(比色法)含量以及有效成分总量率(石斛碱含量×折干率)等指标;采收二年生秋冬季(第2年10月至第3年3月,每月15日采收)金钗石斛茎,测定其折干率和多糖、石斛碱含量。结果:二年生金钗石斛茎的折干率、多糖含量与有效成分总量率较一年生与三年生高,总生物碱、石斛碱含量为一年生(0.52%、0.48%)>二年生(0.48%、0.44%)>三年生(0.32%、0.22%)。从第2年10月至第3年3月,二年生金钗石斛茎的折干率逐月上升,多糖、石斛碱含量均呈现先上升后下降的趋势,其中多糖含量在2月最高(17.32%),石斛碱含量于12月最高(0.51%)。结论:综合植物开花特性、折干率、多糖与石斛碱含量等指标,建议合江金钗石斛最适采收期为二年生的12月和1月(第三年开花前)。
ABSTRACT: OBJECTIVE: To determine quality indexes of Hejiang Dendrobium nobile at different harvesting time and months, and to provide scientific reference for reasonable harvesting period of Hejiang D. nobile. METHODS: The stem of annual, biennial and triennial D. nobile were collected. The drying rate, the contents of polysaccharides (colorimetry), dendrobine (GC) and total alkaloids (colorimetry), effective component total rate (dendrobine×drying rate) were measured. Biennial D. nobile were collected in autumn and winter (Oct. of the second year-Mar. of the third year, 15th day a month) to determine drying rate, the contents of polysaccharides and dendrobine. RESULTS: The drying rate, the contents of polysaccharides and effective component total rate were higher than annual and triennial D. nobile. The content of total alkaloids and dendrobine were in descending order: annual D. nobile (0.52%, 0.48%)>biennial D. nobile(0.48%, 0.44%)>triennial D. nobile (0.32%, 0.22%). From Oct. of the second year to Mar. of the third year, the drying rate of biennial D. nobile was increasing month by month; the contents of polysaccharides and dendrobine increased firstly and then decreased; the content of polysaccharide was the highest in Feb. (17.32%), and the content of dendrobine reached the highest level in Dec. (0.51%).  CONCLUSIONS: The optimal harvesting period is biennial Hejiang D. nobile in Dec. and Jan. (third years before flowering), considering flowering characteristics, drying rate, the contents of polysaccharide and dendrobine.
期刊: 2018年第29卷第1期
作者: 颜寿,赵庭梅,张雪琴,邢嘉懿,胡亚东,淳泽
AUTHORS: YAN Shou,ZHAO Tingmei,ZHANG Xueqin,XING Jiayi,HU Yadong,CHUN Ze
关键字: 合江;金钗石斛;多糖;石斛碱;折干率;气相色谱法;采收月份;生长年限
KEYWORDS: Hejiang; Dendrobium nobile; Polysaccharide; Dendrobine; Drying rate; Gas chromatography;Harvesting month; Growth years
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