甘草提取物对雷公藤甲素损伤后人肝L-02细胞中UGT1A、MRP2表达的影响
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篇名: 甘草提取物对雷公藤甲素损伤后人肝L-02细胞中UGT1A、MRP2表达的影响
TITLE:
摘要: 目的:考察甘草提取物(GE)对雷公藤甲素(TP)损伤后人肝L-02细胞中尿苷二磷酸葡萄糖醛酸转移酶1A(UGT1A)、多药耐药相关蛋白2(MRP2)表达的影响,研究甘草对TP的减毒机制。方法:采用MTT法测定0(空白对照)、40、80、160 nmol/L TP分别培养L-02细胞12、18、24 h后的细胞存活率。将L-02细胞分为空白对照组(空白培养基)、模型对照组(80 nmol/L TP)和GE预处理组(分别以30、60、90 mg/L GE预处理24 h后再加入80 nmol/L TP),继续培养18 h后,采用MTT法检测各组的细胞存活率。在上述分组(GE预处理组的GE浓度为60 mg/L)基础上增设利福平(RIF)组(阳性对照,10 μmol/L RIF预处理24 h后再加入80 nmol/L TP),培养24 h后,检测各组细胞中UGT1A、MRP2蛋白表达。结果:TP对细胞活性的抑制作用与浓度和时间呈正相关。与空白对照组比较,模型对照组的细胞存活率明显降低(P<0.05),细胞中MRP2蛋白表达明显减弱(P<0.01)。与模型对照组比较,30、60、90 mg/L GE预处理组的细胞存活率均明显升高(P<0.01);60 mg/L GE预处理组细胞中UGT1A和MRP2蛋白表达均明显增强(P<0.01)。结论:GE预处理可减轻TP对人肝L-02细胞的损伤,其减毒机制可能与提高细胞中UGT1A、MRP2的表达有关。
ABSTRACT: OBJECTIVE: To investigate the effects of Glycyrrhiza uralensis extract (GE) on the expression of uridine diphosphate glucuronyltransferase 1A (UGT1A) and multidrug resistance associated protein 2 (MRP2) in human liver L-02 cells damaged by triptolide (TP), and to study attenuated mechanism of G. uralensison for TP. METHODS: The survival rates of L-02 cells were determined by MTT assay after cultured with 0 (blank control), 40, 80, 160 nmol/L TP for 12, 18, 24 h. L-02 cells were divided into blank control group (blank culture medium), model control group (80 nmol/L TP) and GE pretreatment group (adding 80 nmol/L TP after pretreated with 30, 60, 90 mg/L GE for 24 h); after cultured for 18 h, survival rates of L-02 cells were determined by MTT assay. Rifampin (RIF) group (positive control, adding 80 nmol/L TP after pretreated with 10 μmol/L RIF for 24 h) was added on the basis of the above grouping (GE concentration of 60 mg/L in GE pretreatment group).After cultured for 24 h, the protein expressions of UGT1A and MRP2 were detected. RESULTS:The inhibition effect of TP on cell proliferation was positively correlated with the concentration and the time. Compared with blank control group, cell survival rate of model control group was decreased significantly (P<0.05), and the protein expression of MRP2 was decreased significantly (P<0.01). Compared with model control group,cell survival rates of 30, 60, 90 mg/L GE pretreatment groups were all increased significantly (P<0.01). The protein expressions of UGT1A and MRP2 were increased significantly in 60 mg/L GE pretreatment group (P<0.01). CONCLUSIONS: GE pretreatment can relieve TP-induced human liver L-02 cell damage, and its attenuated mechanism may be associated with the increase the expression of UGT1A and MRP2.
期刊: 2018年第29卷第1期
作者: 张靖,胡骞,谭亲友,朱胜楠
AUTHORS: ZHANG Jing,HU Qian,TAN Qinyou,ZHU Shengnan
关键字: 甘草提取物;雷公藤甲素;尿苷二磷酸葡萄糖醛酸转移酶1A;多药耐药相关蛋白2;人肝L-02细胞
KEYWORDS: Glycyrrhiza uralensis extract; Triptolide; Uridine diphosphate glucuronyltransferase 1A; Multidrug resistance associated protein 2; Human liver L-02 cells
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