HPLC法测定利尔眠胶囊中3种成分的含量
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篇名: HPLC法测定利尔眠胶囊中3种成分的含量
TITLE:
摘要: 目的:建立同时测定利尔眠胶囊中盐酸小檗碱、盐酸巴马汀、桂皮醛含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent Zorbax SB-C18,流动相为乙腈-0.05 mol/L磷酸二氢钾溶液(23 ∶ 77,V/V),流速为1.0 mL/min,检测波长为345 nm(0~20 min,盐酸小檗碱、盐酸巴马汀)和290 nm(>20~30 min,桂皮醛),柱温为30 ℃,进样量为10 μL。结果:盐酸小檗碱、盐酸巴马汀、桂皮醛进样量检测线性范围分别为0.036 80~0.736 0、0.016 76~0.335 2、0.004 140~0.082 80 μg(r≥0.999 5);检测限分别为0.110 4、0.050 3、0.124 2 ng,定量限分别为0.368 0、0.167 6、0.414 0 ng;精密度、稳定性(12 h)、重复性试验的RSD均<2.0%(n=6);加样回收率为96.16%~99.73%,RSD为0.99%~1.21%(n=6)。结论:建立的方法操作简单、重复性好,适用于同时测定利尔眠胶囊中盐酸小檗碱等3种成分的含量。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of 3 components in Li’ermian capsule, including berberine hydrochloride, palmatine hydrochloride and cinnamaldehyde. METHODS: HPLC method was adopted. The determination was performed on Agilent Zorbax SB-C18 with mobile phase consisted of acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution (23 ∶ 77, V/V) at the flow rate of 1.0 mL/min. The detection wavelengths were set at 345 nm (0-20 min, berberine hydrochloride, palmatine hydrochloride) and 290 nm (>20-30 min, cinnamaldehyde), and column temperature was 30 ℃. The sample size was 10 μL. RESULTS: The linear ranges of berberine hydrochloride, palmatine hydrochloride and cinnamaldehyde were 0.036 80-0.736 0, 0.016 76-0.335 2, 0.004 140-0.082 80 μg (r≥0.999 5). The limits of detection were 0.110 4, 0.050 3,0.124 2 ng, and the limits of quantitation were 0.368 0, 0.167 6, 0.414 0 ng, respectively. RSDs of precision, stability (12 h) and reproducibility tests were all lower than 2.0% (n=6). The recoveries were 96.16%-99.73% (RSD=0.99%-1.21%, n=6). CONCLUSIONS: Established method is simple, reproducible and suitable for simultaneous determination of 3 components such as berberine hydrochloride in Li’ermian capsule.
期刊: 2018年第29卷第1期
作者: 王雷,于荣
AUTHORS: WANG Lei,YU Rong
关键字: 利尔眠胶囊;高效液相色谱法;盐酸小檗碱;盐酸巴马汀;桂皮醛;含量测定
KEYWORDS: Li’ermian capsule; HPLC; Berberine hydrochloride; Palmatine hydrochloride; Cinnamaldehyde; Content determination
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